中药合剂对缺氧性脑损害的抗氧化保护作用

目的观察中药合剂对缺氧大鼠脑组织的抗氧化保护作用。方法中药合剂的主要成分为丹参、当归、党参、黄芪,生药含量0.4 g/ml。以低压舱减压模拟缺氧,分别观察低氧、常氧条件下中药合剂对SD大鼠脑组织谷胱甘肽含量(GSH)、谷胱甘肽过氧化物酶(GSH-PX)和过氧化氢酶(CAT)活性的影响,并检测不同浓度中药合剂在体外的抗羟自由基效力。结果①低氧暴露动物脑组织GSH含量下降,GSH-PX和CAT活性降低(P<0.01)。使用中药合剂后,GSH与GSH-PX显著提高。②常氧状态下,中药合剂就可提高GSH含量与GSH-PX活性。③体外实验显示中药合剂能直接清除H2O2/Co2 体系中产生的羟自由基。结论在该实验条件下,中药合剂对大鼠缺氧脑组织有一定的抗氧化神经保护作用。药物的这种作用与其提高机体自身的抗氧化能力以及其直接的清除自由基作用有关。

Anti-oxidative Protective Effect of Chinese Herbal Mixture on Hypoxic Rat Brain

Objective To observe the effect of Chinese herbal mixture against hypoxic brain in rats.Methods Hypoxic state was established in hypobaric chamber.We investigated the effects of Chinese herbal mixture on content of GSH,activities of GSH-PX and CAT of rat brain in both hypoxic and normoxic states. In another experiment,the hydroxyl radical scavenging activity of above mixture was measured in vitro using alizarin violet spectrophotometry.Results Content of GSH and activities of GSH-PX and CAT all decreased significantly in hypoxic group,but GSH and GSH-PX decreased less with pretreatment of Chinese herbal mixture.In normoxic state,GSH and GSH-PX were elevated with Chinese herbal mixture.In addition,Chinese herbal mixture scavenged hydroxyl radical in in vitro H2O2/Co2 experimental system in a dose-dependent manner.Conclusion The results suggest the mechanisms are related to the Chinese herbal mixture of this study plays an anti-oxidative neuroprotective role on rat brain exposed to hypoxia,and that elevation of the activity of anti-oxidative enzyme GSH-PX and the content of GSH,and a direct free radical-scavenging effect.