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p53蛋白和p53 mRNA在牙源性囊肿和成釉细胞瘤中表达的意义
引用本文:钟鸣,王秀侠,李自娟,王洁. p53蛋白和p53 mRNA在牙源性囊肿和成釉细胞瘤中表达的意义[J]. 中国医科大学学报, 2003, 32(3): 224-225
作者姓名:钟鸣  王秀侠  李自娟  王洁
作者单位:1. 中国医科大学口腔医学院病理学教研室,辽宁,沈阳,110002
2. 沈阳市口腔医院
基金项目:辽宁省教育厅基金资助项目 ( 2 0 12 2 17)
摘    要:目的:探讨成釉细胞瘤(AB)和牙源性角化囊肿(OKC)中p53蛋白及p53 mRNA表达的生物学意义。方法:采用免疫组化S—P法和原位杂交方法分别对p53蛋白和p53 mRNA进行检测。结果:p53蛋白在AB、OKC及正常口腔粘膜上皮细胞核中表达阳性率分别为85.7%、44.0%和33.3%,组间差异显著(P<0.01)。伴随AB的复发与恶变,p53蛋白阳性表达强度增加,核表达数目增多,组间差异显著(P<0.01)。角化细胞颗粒细胞p53蛋白表达减少或缺失,但却有p53 mRNA的阳性产物。p53 mRNA与p53蛋白之间存在高度负相关(r=-0.957,P<0.01)。结论:p53蛋白和p53 mRNA的表达是判断成釉细胞瘤预后的指标。

关 键 词:成釉细胞瘤  牙源性角化囊肿  p53基因  免疫组化  原位杂交
文章编号:0258-4646(2003)03-0224-02
修稿时间:2002-10-08

Expression of p53 mRNA in Odontogenic Cysts and Tumors
ZHONG Ming,WANG Xiu-xia,LI Zi-juan,WANG Jie. Expression of p53 mRNA in Odontogenic Cysts and Tumors[J]. Journal of China Medical University, 2003, 32(3): 224-225
Authors:ZHONG Ming  WANG Xiu-xia  LI Zi-juan  WANG Jie
Affiliation:ZHONG Ming1,WANG Xiu-xia2,LI Zi-juan1,WANG Jie1
Abstract:Objective: Our aim was to study the expression of p53 mRNA and p53 protein in amleoblastoma (AB) and odontogenic keratocyst (OKC). Methods: The p53 mRNA and protein were determined with S-P method and in situ hybridization. Results: The positive rates of p53 protein in AB, OKC, and normal oral mucosa were 85.7%, 44.0% and 33.3%. There were statistical differences among groups (P<0.01). Accompanied with the recurrence and transformation, the positive rates of p53 protein and the number of nuclei with positive expression of p53 potein were increased. The differences among groups were significant (P<0.01). In keratinocyte and granule cell, the expression of p53 protein was low or negative, but that of p53 mRNA was positive. The relationship between p53 mRNA and p53 protein was highly negative (r=-0.957, P<0.01). Conclusion: The expressions of p53 mRNA and p53 protein are important signs to evaluate the prognosis of AB.
Keywords:ameloblastoma  odontogenic keratocyst  p53 gene  immunohistochemistry  in situ hybridization
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