DPC4 基因在肺癌中的表达及其与微血管形成和凋亡的关系

 目的 探讨DPC4（deleted in pancreatic carcinoma locus 4，DPC4）基因在非小细胞肺癌（nonsmall cell lung carcinoma，NSCLC）中的表达及其与微血管形成和凋亡的关系。方法 利用免疫组织化学S-P法检测52例NSCLC组织、19例相应的癌旁正常肺组织中DPC4、VEGF的表达，用CD34标记血管内皮细胞并计算微血管密度MVD值，应用TUNEL技术对细胞凋亡情况进行了检测并计算凋亡指数。结果 DPC4在肺癌原发灶中的阳性表达率为63．5％（33／52），与癌旁正常肺组织中的阳性表达率89．5％（17／19）相比，DPC4阳性表达水平显著降低（P〈0．05）DPC4与组织学类型、肿瘤细胞分化程度无关（P〉0．05），但与淋巴结转移显著相关（P〈0．05）。52例NSCLC中，DPC4的表达与VEGF、MVD值均呈负相关（r=-0．303，P=0．020）。DPC4阳性组凋亡指数（apoptotic index，AI）值明显高于DPC4阴性组（P〈0．05）。结论 DPC4的低表达可能是肺癌发生过程的早期事件，并可通过直接或间接的作用促进肺癌血管生成，从而促进肺癌的淋巴结转移，DPC4的高表达可能促进细胞的凋亡。

Expression of DPC4 Gene in Non-Small Cell Lung Carcinoma and Its Relation to Angiogenesis and Apoptosis

Objective To study the expression of DPC4(deleted in pancreatic carcinoma locus 4,DPC4)in non-small cell lung carcinoma as well as its association with angiogenesis and apoptosis. Methods The expression of DPC4, VEGF and CD34 was detected in 52 cases with primary NSCLC and 19 adjacent normal lung tissues by using immunohistochemistrical S-P method.Apoptosis of NSCLC cells was detected by TUNEL technique. Results Positive expression rate of DPC4 in primary lung cancer tissues was 63.5%(33/52),compared with the positive rate 89.5%(17/19)in adjacent normal lung tissues, the DPC4 expression level apparently degraded and there was significant differentiation(P< 0.05).The positive expression of DPC4 had no correlation with tissue types and cellular differentiation(P> 0.05),but it was closely associated with lymph node metastasis(P< 0.05).In 52 patients with NSCLC,the relationships between DPC4 and VEGF, and DPC4 and MVD,showed apparently negative correlation(r= -0.303, P = 0.020).The AI in DPC4-positive group was much higher than that in DPC4-negative group. Conclusion The low expression of DPC4 could be an early event during the course of the NSCLC's formation. Simultaneously,it also promoted lymph node metastasis by promoting the angiogenesis in NSCLC derectly or indirectly.The high expression of DPC4 may promote cell apoptosis.