白头翁汤对溃疡性结肠炎模型小鼠肠道菌群及短链脂肪酸的影响

目的 探究白头翁汤对溃疡性结肠炎(Ulcerative colitis,UC)模型小鼠的治疗作用及可能机制.方法 采用葡聚糖硫酸钠(Dextran sulphate sodium,DSS)诱导UC模型小鼠,给予白头翁汤干预.实验过程中,每日记录小鼠体质量、腹泻、便血情况.实验结束后测量小鼠结肠长度,HE染色观察结肠病理...

Baitouweng Decoction Alleviates DSS-Induced Colitis via Rebalancing Gut Microbiota and Regulating Short Chain Fatty Acids

OBJECTIVE  To explore the treatment and mechanism of Baitouweng decoction on ulcerative colitis mice.METHODS  Colitis mice were induced by DSS and treated with or without Baitouweng decoction. During experiment, body weight of mice, the symptom of diarrhea and bloody stool were recorded. At the end of experiment, the length of colon was measured, colonic pathology was observed after HE staining, the expression of cytokines was evaluated by qPCR, the construction of gut microbiota was detected by 16S rRNA, the concentration of short chain fatty acids was measured by gas chromatography-mass spectrometry.RESULTS  Compared with the healthy mice, the body weight and the length of colon decreased in colitis mice(P < 0.01), the construction of colon was disorder with infiltration of inflammation cells, and the expressions of IL-1β, IL-6 and TNF-α was upregulated(P < 0.05, P < 0.01). But the expression of anti-inflammatory cytokines were not different between these two groups. Moreover, dysbacteriosis and low level of short chain fatty acids were observed. After Baitouweng decoction treatment, the weight of mice and the length of colon were increased(P < 0.05, P < 0.01). The expressions of pro-inflammatory cytokine, such as IL-1β, IL-6 and TNF-α, was downregulated(P < 0.05, P < 0.01). And the construction of gut microbiota was rebalanced especially in DSS+BTW-H group. The contents of butyric acid and valeric acid increased significantly(P < 0.05, P < 0.01).CONCLUSION  Baitouweng decoction has therapeutic effect on DSS-induced colitis through rebalancing gut microbiota and short chain fatty acids.