针刺“后三里”穴不同留针时间对小鼠甩尾痛阈的影响

目的:通过观察针刺"后三里"穴对小鼠热水甩尾痛阈的影响,探讨留针时间与针灸效应的关系.方法:健康小鼠24只,随机分为针刺即刻组(A组)、留针10 min组(B组)、留针20 min组(C组)、留针30 min组(D组),每组6只.手针针刺小鼠"后三里"穴后,以50℃热水浸鼠尾,分别记录针刺即刻、留针10 min、留针20 min、留针30 mind、鼠甩尾潜伏期,对比鼠尾入水至甩尾出水的时间间隔.结果:A、C两组针刺"后三里"能显著提高小鼠的甩尾痛阈(均P<0.01),而B、D两组针刺"后三里"对小鼠的甩尾痛阈提高不显著(均P>0.05).留针30 min内针刺镇痛效应呈波浪形曲线,留针20 min小鼠甩尾潜伏期达到最大值,即针刺达到最大镇痛效果.结论:手针镇痛的留针时间以20 min为最佳.

Effect of needling "Housanli" (ST 36)with different retaining-needle time on the pain threshold of mice using the hot water tail-flick test

Objective To observe the effect of needling "Housanli" (ST 36) with different retaining-needle time on the pain threshold of mice using the hot water tail-flick test, and investigate the relationship between the retaining-needle time and the therapeutic effect. Methods Twenty-four healthy mice were randomly divided into four groups: no retaining-needle group (group A), retaining-needle 10 min group (group B) , retaining-needle 20 min group (group C) and retaining-needle 30 min group (group D) , with 6 mice for each group. After acupuncture "Housanli", the tail of mouse was put into the hot water at 501, and the intervening time from the tail entered water to the tail flicked out of water was recorded and analyzed for each group. Results After acupuncturing "Housanli", the pain threshold was significantly improved with the hot water tail-flick test in both group A and group C (both P<0. 01) , however, there was no significant difference in both group B and group D (both P>0. 05). Within 30 min of retaining-needle, the effect curve of acupuncture analgesia was showed in a waved line. In contrast, the maximum value of latent period on the tail-flick was detected in the group C that was the biggest effect of acupuncture analgesia among the four groups. Conclusion The best retaining-needle time of hand-acupuncture for acupuncture analgesia is 20 min.