| 层粘连蛋白对人胃癌细胞内游离钙及钙调蛋白和核DNA含量的影响 |
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| 引用本文： | 肖军军,孙梅,张页.层粘连蛋白对人胃癌细胞内游离钙及钙调蛋白和核DNA含量的影响[J].北京大学学报(医学版),1994(2). |
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| 作者姓名： | 肖军军 孙梅 张页 |
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| 作者单位： | 北京医科大学细胞生物学教研室 |
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| 摘 要： | 本文研究外源性层粘连蛋白与人胃癌细胞膜上其受体介导细胞内Ｃａ２＋浓度及钙调蛋白（ＣａＭ）和ＤＮＡ发生的变化。结果表明，层粘连蛋白可引起细胞内Ｃａ２＋浓度升高和钙调蛋白含量显著升高。同时伴有核ＤＮＡ含量增加。提示外源性层粘连蛋白与质膜上的层粘连蛋白受体结合后可能通过细胞内Ｃａ２＋影响核内ＣａＭ，进而促进ＤＮＡ的合成。该信息通路在层粘连蛋白促进肿瘤细胞粘着，铺展，增殖等生物学效应中可能起重要调节作用。
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| 关 键 词： | 层粘连蛋白，游离钙，钙调蛋白，DNA，人胃癌细胞 |
THE VARIATION IN CYTOSOLIC FREE CAICIUM,NUCIEOUS CALMODULIN AND DNA
OF HUMAN GASTRIC CANCER CELLS UNDER THE ACTION OF LAMININ |
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| Institution: | Department of Cell Biology Xiao Junjun,et al |
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| Abstract: | hanges in the
content of cytosolic Ca2+ and the nucleous calmodulin and DNA of
human gastriccancer(BGC-823 )cells bound with and without external
laminin(LN)were studied using a cytofluor- ophotometer The followIng
results were obtained:Green annulus can be seen on the membrane of
ca- ncer cells.When cells cultured on the LN coated glass surface,the
content of cytosolic Ca2+ and the nucleous calmodulin increased
obviously,accopanied by increase of DNA,which suggesrs that the cyto-
solic Ca2+ may play a role of second messenger by means of CaM in the
biological progress of LN promoting cancer cell proliferation |
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| Keywords: | Laminin Cytosolic free calcium Calmodulin Nuclear DNA Human gastric cancer cell line |
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