Microactuator device for integrated measurement of epithelium mechanics |
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Authors: | Vikram Mukundan W James Nelson Beth L Pruitt |
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Institution: | 1. Department of Mechanical Engineering, Stanford University, Stanford, CA, USA 3. Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany 2. Department of Biology, Stanford University, Stanford, CA, USA
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Abstract: | Mechanical forces are among important factors that drive cellular function and organization. We present a microfabricated device with on-chip actuation for mechanical testing of single cells. An integrated immersible electrostatic actuator system is demonstrated that applies calibrated forces to cells. We conduct stretching experiments by directly applying forces to epithelial cells adhered to device surfaces functionalized with collagen. We measure mechanical properties including stiffness, hysteresis and visco-elasticity of adherent cells. |
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