"促愈瘢痕消"对兔耳增生性瘢痕作用的实验研究

目的：探讨“促愈瘢痕消”对兔耳增生性瘢痕的作用。方法：用新西兰白兔12只，均在右侧兔耳腹侧面制作增生性瘢痕动物模型。待瘢痕形成后随机分为两组，每组6只：试验组（A组）外用“促愈瘢痕消”，对照组（B组）外用生理盐水，均1次／d，连用1月。分别在瘢痕形成时、治疗后4周测量瘢痕厚度，治疗后1、4周切取瘢痕组织做组织病理学及p53、增殖细胞核抗原（proliferating cell nuclear antigen,PCNA）测定。结果：A组在4周时较B组瘢痕厚度明显变薄，颜色变浅，质地变软，胶原色淡而排列稀疏，随用药时间延长差异明显；两组间瘢痕厚度差异有统计学意义（P＜0.05）；p53、PCNA阳性表达率在1、4周时两组间差异有统计学意义（P＜0.05），A组在1、4周时差异有统计学意义（P＜0.05），B组在1、4周时差异无统计学意义（P＞0.05）。结论：“促愈瘢痕消”能够明显抑制兔耳瘢痕的增生，且机理可能是减少p53及PCNA的表达，有望成为治疗增生性瘢痕的有效药物。

The effect of Cuyubanhenxiao on hypertrophic scars in rabbit ears

Objective: To explore the effect of Cuyubanhenxiao on hypertrophic scars in rabbit ears.Methods: Twelve adult New Zealand white rabbits were given hypertrophic scars over the ventral surface of the right ears and were randomly divided into the Cuyubanhenxiao group(group A) and the control group(group B).They were subjected to the drugs once every day for 1 month.The thickness of the scars was determined at the formation and at 4 weeks after treatment.Immunohistochemical(P53 and PCNA) and histomorphology were used to determine the scar tissues at 1 and 4 weeks.Results: The thickness of the scar was significantly different between the two groups(P<0.05).Expression of p53 and PCNA was significantly different between the 1 week and 4 weeks groups(P<0.05).Expression of p53 and PCNA was significantly different in group A between the 1 week and 4 weeks groups(P<0.05),but there was no significant difference in group B between the 1 week and 4 weeks groups(P>0.05).Conclusions: Cuyubanhenxiao can heal hypertrophic scars of rabbit ears,which may be caused by the decrease of the expression of p53 and PCNA.