高功率微波辐照后大鼠睾丸生精细胞Bc1-2和C-myc蛋白表达改变

目的: 检测高功率微波(HPM)与细胞凋亡相关蛋白Bc1 2和C myc的关系。　方法: 健康成年雄性SD大鼠 125只,随机分为 25组,每组 5只。以S波段平均功率密度分别为 10、20mW/cm2的HPM辐照实验组大鼠 5、10min,分别于照后 6、24、48、72、120h取睾丸组织,应用免疫组化SP法检测Bc1 2和C myc蛋白表达改变。对照组不经HPM辐照。　结果: HPM辐照后, 24h组Bc1 2和C myc蛋白表达升高, 20mW/cm2组两者升高较 10mW/cm2组升高明显,差别有显著性(P<0. 01),正常对照组无表达。　结论: HPM辐照大鼠后 24h,促进Bc1 2和C myc蛋白的活化和表达,可能是HPM诱导凋亡的机制之一。

Effects of High Power Microwave on the Expressions of Bcl-2 and C-myc Proteins in the Rat Testis

Objective: To investigate the changes in the expressions of Bcl-2 protein and C-myc protein in the spermatogenic cells of rats after exposure to high power microwave (HPM) and to elucidate the possible mechanisms underlying the apoptosis induced by HPM at the genetic translation level. Methods: One hundred and twenty-five healthy male SD rats were divided randomly into unexposed control and experimental groups. The latter were radiated with S wave band 10 W/cm 2 , 20 W/cm 2 HPM for 5 min and 10 min. Testicular samples were taken 6 h , 24 h, 48 h, 72 h and 120 h after radiation and studied respectively. Five blank radiation groups served as controls. Then immunohistochemical SP staining was performed to test the expressions of Bcl-2 protein and C-myc protein in the spermatogenic cells in the rats. Results: The expression of Bcl-2 protein in the 24 h group was up-regulated after radiated for 5 minutes and 10 minutes by HPM , higher in the 20 mW/cm 2 group than in the 10 mW/cm 2 group(P< 0.01). There was no expression of C-myc/Bcl-2 protein in the control group. Conclusion: Exposure to HPM for 24 h can up-regulate the expressions of C-myc protein and Bcl-2 protein in the spermatogenic cells of rats, which might be one of the mechanisms of the apoptosis induced by HPM.