人CD200基因克隆及pcDNA3-CD200表达质粒的构建

目的：克隆人CD200基因并构建pcDNA3-CD200表达质粒。方法：采用逆转录聚合酶链式反应（RT-PCR）法，从用200 μg Con A刺激62 h后的正常人外周血白细胞中扩增出CD200基因，与pMD18T载体连接后，做全自动DNA测序，并用亚克隆的方法构建pcDNA3-CD200表达质粒。结果：从正常人外周血白细胞扩增的人CD200基因与GenBank中人CD200序列（NM -005944）完全相同。结论：成功地克隆人CD200基因并构建了pcDNA3-CD200表达质粒。

Cloning of human CD200 gene and construction of expression plasmid pcDNA3-CD200

Objective To clone the human CD200 gene and to construct the expression plasmid pcDNA3-CD200. Methods The CD200 gene was obtained with the technique of RT-PCR from human peripheral white cells at 62 h after stimulation with 200 μg Con A.With automatic sequencing of pMD18T-CD200, the pcDNA3-CD200 recombinant plasmid was constructed by subclone, CD200 gene was obtained from human peripheral white cell. Results The obtained CD200 gene was completely consistent with the human CD200 sequence in GenBank (NM 005944). Conclusion The human CD200 gene is successfully cloned and pcDNA3-CD200 recombinant plasmid is constructed.