基于非靶向代谢组学的槲蕨代谢产物分析

目的 分离鉴定槲蕨不同部位中功能成分并为其代谢产物生物合成途径提供重要信息。方法 采用非靶向代谢组对槲蕨的根茎、叶柄、叶的代谢组分进行高度富集比较分级。结果 在根茎、叶柄和叶中共鉴定出493个代谢物，通过多元统计分析筛选出98个差异代谢物（DAMs）。其中，四甲氧基黄酮代谢物在槲蕨的叶柄中表达最高，显著高于叶；芹菜素代谢物在槲蕨的叶中表达最高，显著高于根茎和叶柄；其余13个黄酮类代谢物均集中在槲蕨的根茎中，显著高于其他部位。根茎、叶柄和叶的DAMs主要富集在淀粉和蔗糖代谢通路。结论 可为槲蕨不同部位中功能成分的分离鉴定和代谢生物合成途径的解析提供参考。

Analysis of Metabolites of Drynaria roosii Nakaike Based on Non-targeted Metabonomics

Objective To provide important information for separating and identifying functional components in different tissue of Drynaria roosii Nakaike and for elucidating biosynthesis pathways of metabolites of D. roosii.Methods The non-targeted metabolomics was performed for comparing and grading metabolic components of the rhizome, petiole, and leaf of D. roosii in terms of high enrichment.Results A total of 493 metabolites were identified in the rhizome, petiole, and leaf, and 98 differential metabolites were selected by the multivariate statistical analysis, Among them, the expression of tetramethoxy flavone in the petiole of D. roosii was the highest, significantly higher than that in the leaf; the expression of apigenin in the leaf of D. roosii was the highest, significantly higher than that in the rhizome and petiole; the other 13 flavonoid metabolites were concentrated in the rhizome of D. roosii, significantly higher than other parts. Differential metabolites of the rhizome, petiole, and leaf were mainly concentrated in the pathway of starch and sucrose metabolism.Conclusion This study provides a reference for separating and identifying functional components in different tissue of D. roosii and for elucidating biosynthesis pathways of metabolites.