鸭嘴花碱经口单次给药安全性评价研究

目的：通过研究鸭嘴花碱急性毒性,评价鸭嘴花碱的安全性。方法：将120只昆明种小鼠,雌雄各半,随机均等分为6组,每组20只。按40 mL·kg^-1小鼠灌胃体积给予相应剂量的药物,密切观察4 h, 记录小鼠毒性反应与死亡率。连续观察14天,记录小鼠一般状态,解剖观察脏器并计算脏器指数,对存活小鼠肝脏、肾脏进行HE染色和生化指标检测。结果：小鼠对鸭嘴花碱最大耐受量（MTD）为153.6 mg·kg^-1。观察14天后,与空白组比较,小鼠一般状态、脏器指数无明显变化;病理结果显示无肝肾毒性;血浆中ALT、Cre无显著变化,AST、BUN含量有下降趋势。结论：小鼠对鸭嘴花碱的MTD为153.6 mg·kg^-1,该剂量下对小鼠安全性高。

Study on Safety Evaluation of Single Oral Administration of Vasicine

Objective: To evaluate the safety of vasicine by studying its acute toxicity. Methods: A total of 120 Kunming mice were randomly divided into 6 groups with 20 mice in each group. The corresponding dose of the drug was administered at 40 mL·kg^-1 mouse intragastric volume. The toxic reaction and mortality of mice were observed closely for 4 h. The mice were observed continuously for 14 days and their general state was recorded. The organs were dissected and the organ index was calculated. HE staining and biochemical indicators on the livers and kidneys of surviving mice were detected. Results: The maximum tolerated dose (MTD) of vasicine in mice was 153.6 mg·kg^-1. After 14 days of observation, compared with blank group, the general state and organ index of mice had no significant change. Pathological results showed no hepatorenal toxicity. There were no signifi cant changes in ALT and Cre in plasma, while the contents of AST and BUN decreased. Conclusion: The MTD of mice was 153.6 mg·kg^-1, which was safe for mice.