野生抽薹与未抽薹防风中色原酮类成分比较研究

目的建立防风HPLC指纹图谱,并测定防风中4种色原酮含量的方法,比较抽薹防风与未抽薹防风的异同。方法采用Agilent Ecilpse Plus C18色谱柱(4.6 mm×250 mm,5μm),柱温为35℃,以甲醇-水为流动相梯度洗脱,流速为1.0 mL·min^-1;以DAD检测器检测,检测波长为254 nm。结果防风指纹图谱中有10个共有峰,6批抽薹防风与未抽薹防风相似度都在0.94以上,2批二茬防风的相似度低于0.90。本方法对升麻素苷、升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷线性关系良好,相关系数在0.9984~0.9998之间;加样回收率(n=6)分别为95.05%,98.60%,98.30%和99.02%,重复性实验的RSD分别为1.60%、1.77%、1.24%和3.09%。抽薹防风与未抽薹防风比较,升麻素抽薹后升高,其他3种色原酮类成分无明显变化趋势,4种色原酮类成分的总量亦无明显变化。结论构建的防风HPLC指纹图谱结合多成分含量测定方法,为完善防风的质量控制和品质评价提供了更全面的参考,抽薹防风与未抽薹防风无明显区别,二茬防风与一茬防风差别显著。

Comparison of Chromone Components and Their Contents between Bolting and Non-bolting Saposhnikoviae Radix

OBJECTIVE To establish a high performance liquid chromatography(HPLC)method for determining chromone components in Saposhnikoviae Radix,and compare the similarities and differences between bolting and non-bolting samples.METHODS Agilent Ecilpse Plus C18 column(4.6 mm×250 mm,5μm)was used for chromatographic analysis,water and methanol were used as mobile phase for gradient elution at a flow rate of 1 mL·min^-1.Detection was conducted at 254 nm.The column temperature was maitained at 35℃and the injection volume was 10μL.RESULTS There were 10 common peaks in the HPLC fingerprints of 8 batches of Saposhnikoviae Radix,with similarity above 0.94 in six batches of bolting and non-bolting samples and below 0.90 in two batches of second-stubble samples.The HPLC assay had good linearity for the four chromone components.The average recoveries for prim-O-glucosylcimifugin,cimicifugin,5-O-methylvisamidol glycoside and sec-O-glu cosylhamaudol were 95.05%,98.62%,98.3%and 99.02%,and the RSDs of repeatability test were 1.60%,1.77%,1.24%and 3.09%,respectively.When comparing the bolting and non-bolting Saposhnikoviae Radix,the content of cimicifugin increased after bolting,the other three components and the total amount of the four chromone components did not have significant variation.CONCLUSION The establishment of HPLC fingerprint combined with simultaneous determination of four chromone components provides a more comprehensive reference for the quality control and quality evaluation of Saposhnikoviae Radix.There are no obvious differences in chromone components between bolting and non-bolting Saposhnikoviae Radix,but there is significant difference between second-stubble and first-stubble Saposhnikoviae Radix.