我国部分地区呼吸道合胞病毒地方株磷蛋白基因序列分析

目的 了解我国呼吸道合胞病毒（ＲＳＶ）地方株的Ｐ（Ｐｈｏｓｐｈｏｒｐｒｏｔｅｉｎ，Ｐ）蛋白基因状况和变异特征。方法 选取不同地区具有不同流行特征的６株ＲＳＶ地方株，以提取的病毒ｍＲＮＡ为模板进行逆转录－聚合酶链扩增（ＲＴ－ＰＣＲ）扩增、目的基因的克隆及序列测定分析。结果 地方株和同亚型原型株之间Ｐ蛋白核苷酸序列及推导出的氨基酸序列同源性均超过９５％；不同亚型间存在较大差异，核苷酸全序列同源性低于８５％，尤其在３′端非编码区及中间变异区。由氨基酸序列推导出的疏水性分析图显示，Ａ、Ｂ亚型中间变异区存在疏水性的不同，这为解释Ａ、Ｂ亚型间Ｐ蛋白电泳迁移率的不同提供了一种可能。结论 我国ＲＳＶ地方株与原型株之间的Ｐ蛋白无论在核苷酸水平还是在氨基酸水平均存在一定的变异。这些结果对于了解我国ＲＳＶ地方株的基因状况提供了有益的

Sequence analysis of phosphoprotein genes of respiratory syncytial virus field strains isolated in China

OBJECTIVE: To get information about phosphoprotein (P) genes of RSV field strains isolated in China. METHODS: The complete nucleotide sequences of the P protein genes from six respiratory syncytial virus strains were determined with cDNA clones which had been amplified by RT-PCR and cloned into PTZ18R plasmid vector and compared with those of previously published counterparts of A2 and CH18537 strains. RESULTS: The data indicated that there had high identities (>95%) at nucleotide and amino acid levels among the strains within the same subgroup; there were more divergence among strains (<85%) from different subgroups at nucleotide and amino acid levels of P genes, especially in the 3'-noncoding regions and the divergent domains. The analysis of hydrophobicity indicated that there had difference from 50 to 100 amino acid of P proteins between A and B subtypes. CONCLUSIONS: The analysis of the P genes confirm that there existed variability among RSV field strains and strains A2 and CH18537. The data described above revealed the characteristics of the P genes of RSV field strains isolated in China.