深圳市HIV-1 E亚型感染毒株的分子流行病学分析

目的 了解人类免疫缺陷病毒１型Ｅ亚型毒株在深圳市不同人群中流行传播情况、流行时间和传播规律。方法 应用聚合酶链反应（ＰＣＲ）方法对１９９６年深圳市检出的３份人类免疫缺陷病毒１型（ＨＩＶ－１）感染者外周血单个核细胞样本进行扩增，获得ＨＩＶ－１膜蛋白（ｅｎｖ）基因片段，并对Ｃ２－Ｖ３及其邻区３５０～４５０个核苷酸序列进行测定和分析。结果 这３份血样为ＨＩＶ－１Ｅ亚型毒株感染（ｓｚ－Ｅ），彼此间的基因离散率为２．６％；与Ａ－Ｅ国际参考亚型及国内部分地区流行的ＢＥ型代表株比较，ｓｚ－Ｅ与Ａ－Ｄ参考亚型共享序列及国内Ｂ亚型代表株间的基因离散率均大于２４％，而与主要代表泰国Ｅ亚型（Ｅｃｏｎ）间的基因离散率仅为６．２％。系统树分析显示，ｓｚ－Ｅ与Ｅｃｏｎ聚集在一起，远离其他国际亚型毒株序列。结论 ＨＩＶ－１Ｅ亚型在深圳的流行

Molecular epidemiology analysis of HIV-1 subtype E strains found in Shenzhen

Objective To investigate the prevalent status of HIV 1 subtype E epidemic among different populations in Shenzhen. Methods HIV 1 proviral DNA. from peripheral blood mononuclear cells (PBMCs) of 3 HIV infected individuals found in Shenzhen was amplified by nested PCR. The C2 V3 and its flanking region (350 450 nucleotides) of HIV 1 env gene were sequenced directly from the PCR products and analysed.Results These 3 cases were confirmed to be infected with HIV 1 subtype E strain (sz E). The innergroups distance of sz E was 2.6%. In comparison with the consesus sequence of intenational A E subgroups and some of subtype B epidemic in China, the genetic distances among sz E and each of A D subgroups and some of subtype B were more than 24%. Furthermore, the phylogenetic tree analysis showed that sz E clustered closely to Econ.Conclusion These findings suggested that the epidemic time for HIV 1 subtype E , which was mainly prevalent among IDUs population and some professional blood donors, had been about 2 3 years in Shenzhen.