| 高效液相色谱法测定人血浆中霉酚酸的浓度 |
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| 引用本文： | 胡咏华,裴奇. 高效液相色谱法测定人血浆中霉酚酸的浓度[J]. 中南药学, 2009, 7(2): 117-119 |
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| 作者姓名： | 胡咏华 裴奇 |
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| 作者单位： | 1. 长沙市第一医院药剂科,长沙,410011
2. 中南大学湘雅三医院药剂科,长沙,410013 |
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| 摘 要： | 目的建立用高效液相色谱测定人血浆中霉酚酸浓度的方法。方法采用SUPELCOSIL C18色谱柱（4.6mm×250 mm,5μm） 流动相：乙腈-0.01 mol.L-1磷酸二氢钾（pH=3.0）溶液（40∶60,v/v） 流速：1.0mL.min-1 检测波长：254 nm。结果霉酚酸在0.4~51.2 mg.L-1线性关系良好（r=0.999 3）,最低检测浓度为0.4 mg.L-1,霉酚酸低、中、高浓度检测的日内、日间RSD分别为2.22%、5.15%、4.13%和1.75%、6.75%、3.35%。结论该法具有操作简便可靠、准确、稳定性高等特点,适合应用于霉酚酸的治疗药物监测和药动学研究。
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| 关 键 词： | 霉酚酸 血药浓度 高效液相色谱法 |
HPLC determination of mycophenolic acid in human plasma |
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| HU Yong-hua,PEI Qi. HPLC determination of mycophenolic acid in human plasma[J]. Central South Pharmacy, 2009, 7(2): 117-119 |
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| Authors: | HU Yong-hua PEI Qi |
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| Affiliation: | 1. First People＇s Hospital of Changsha, Changsha 410011; 2. Department of Phar macy, Third Xiangya Hospital of Central South University, Changsha 410013) |
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| Abstract: | Objective To develop an HPI.C method for the determination of mycophenolic acid in human plasma. Methods The SUPEILCOSIL C18 column （4.6 mm× 250 mm, 5 μm） was used with acetonitrile-0.01 mol · L-1 KH2PO3 （pH=3.0） （40 ： 60, v/v） as the mobile phase. The flow rate was 1.0 mL · min-1 and the detective wavelength was 254 nm. Results The calibration curve of mycophenolic acid was linear at 0. 4-51.2 mg ·L-1 （r= 0. 999 3）. The low limit of determination was 0.4 mg· L -1. The intra-day and inte〉day RSD was 2.22%, 5.15%, 4.13%; and 1.75%, 6.75% and 3.35%. respectively. Conclusion This method is simple, precise, stable and suitable for therapeutic drug monitoring of mycophenolic acid and its pharmacokinetic study. |
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| Keywords: | mycophenolic acid plasma concentration HPLC |
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