体外培养成熟技术对未成熟卵母细胞冻融的影响

目的:探索影响未成熟卵母细胞冻融技术的关键因素。方法:A组:来源于体外受精-胚胎移植(IVF-ET)周期中自愿捐献的多余的成熟卵母细胞(MⅡ期),共56个;B组:来源于手术切除的卵巢组织中的未成熟卵母细胞(GV或MⅠ),共67个。不同成熟时期卵母细胞经慢冻快融后培养和体外受精,观察其体外成熟、受精及胚胎发育情况。结果:A组与B组相比,两组冻融后的存活率有显著性差异(60.71%vs 77.61%,P<0.05);A组受精率高于B组,但两组比较无显著性差异(61.76%vs 50.00%,P>0.05);A组与B组相比,两组冻融后的卵裂率和优质胚胎率有显著性差异(76.19%vs 37.50%,47.62%vs 12.50%,P<0.05)。A组获2枚囊胚,而B组没有囊胚培养成功。结论:体外成熟培养技术可能是影响冻融后未成熟卵母细胞发育的关键因素。

Effect on the developmental potential of the immature cryopreservation oocyte by the technique of maturation in vitro

Objective:To study the effect on the developmental potential of human frozen-thawed immature oocytes by the technique of in vitro maturation.Methods:Fifty six mature oocytes(MⅡ),Group A,were collected from the patients undergoing in vitro fertilization and embryo transfer treatment cycles.Sixty seven immature oocytes(GV/MⅠ),Group B,were collected from ovarian tissue of patients undergoing ovary resection.All the oocytes were cryopreserved using slow cooling method.The surviving immature oocytes of Group B were matured in vitro.Compared the frozen-thawed oocytes' developmental potential of two groups.Results:A significant difference was found in the rate of oocytes' survival between Group A and Group B(60.71% versus 77.61%,P<0.05).The fertilization rate of Group A was higher than that of Group B(61.76% versus 50.00%),but there were no significant differences between two groups(P>0.05).The rates of oocytes cleavage and high-quality embryos of Group A were higher than that of Group B(76.19% versus 37.50%,47.62% versus 12.50%,respectively),there were significant differences between two groups(P<0.05).Succeeding in blastocyst culture derived from frozen-thawed human mature oocytes.Conclusion:The technique of in vitro maturation may be the key factor of influence frozen-thawed immature oocytes development.