Combined toxicity of silica nanoparticles and methylmercury on cardiovascular system in zebrafish (Danio rerio) embryos |
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| Affiliation: | 1. Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China;2. Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China;3. Department of Chemistry, Renmin University of China, Beijing 100872, PR China;1. Toxicology Graduate Program, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada;2. Department of Soil Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada;3. Department of Veterinary Biomedical Sciences, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK S7N 5B4, Canada;1. Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China;2. Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China |
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| Abstract: | This study was to investigate the combined toxicity of silica nanoparticles (SiNPs) and methylmercury (MeHg) on cardiovascular system in zebrafish (Danio rerio) embryos. Ultraviolet absorption analysis showed that the co-exposure system had high absorption and stability. The dosages used in this study were based on the NOAEL level. Zebrafish embryos exposed to the co-exposure of SiNPs and MeHg did not show any cardiovascular malformation or atrioventricular block, but had an inhibition effect on bradycardia. Using o-Dianisidine for erythrocyte staining, the cardiac output of zebrafish embryos was decreased gradually in SiNPs, MeHg, co-exposure groups, respectively. Co-exposure of SiNPs and MeHg enhanced the vascular endothelial damage in Tg(fli-1:EGFP) transgenic zebrafish line. Moreover, the co-exposure significantly activated the oxidative stress and inflammatory response in neutrophils-specific Tg(mpo:GFP) transgenic zebrafish line. This study suggested that the combined toxic effects of SiNPs and MeHg on cardiovascular system had more severe toxicity than the single exposure alone. |
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| Keywords: | Silica nanoparticles Methylmercury Combined toxicity Cardiovascular system Zebrafish embryos |
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