Intra- and inter-rater reliability of ultrasound measures of the anterior cruciate ligament

Diagnostic ultrasound has accurately and reliably been utilized by clinicians to determine ACL morphology at the tibial insertion site, specifically measuring the entire ACL diameter, the anteromedial bundle (AM), and the posterolateral bundle. However, intra- and inter-rater reliability of these measures in a research setting is unknown. The purpose of this study was to determine intra- and inter-rater reliability of ultrasound measures of ACL diameter and AM diameter in researchers with low-to-moderate ultrasound experience. We hypothesized that intra- and inter-rater reliability of ACL and AM diameters would reach acceptable levels, a minimal intraclass correlation (ICC) of 0.6 and a desired ICC of 0.8 with an α of 0.05 and β of 0.20. Fourteen volunteers participated in this study. During the ACL ultrasound measures, participants were seated with their knee flexed to at least 90°. Each rater recorded two images of both the right and left ACL and AM bundles. Next, participants were re-examined by rater one for intra-rater reliability analyses. Two-way random ICCs were conducted for intra-rater (between sessions) and inter-rater reliability for both the full ACL and the AM bundle diameters. Standard errors between sessions for Rater 1’s AM bundle and ACL diameters were less than 0.03 cm. Intra-rater reliability was higher in AM bundles compared to full ACL, 0.76 versus 0.59, respectively. Standard errors between Rater 1 and Rater 2 were less than 0.03 cm. Inter-rater reliability was higher in AM bundles compared to full ACL, 0.71 versus 0.41, respectively. The results of the study indicate researchers with low-to-moderate training with ultrasound measures can locate and measure the ACL, but with greater reliability using the AM.