| 淫羊藿苷对人胚肺二倍体成纤维细胞衰老的干预作用研究 |
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| 引用本文： | 张素琴,周建华,胡蓉. 淫羊藿苷对人胚肺二倍体成纤维细胞衰老的干预作用研究[J]. 老年医学与保健, 2019, 25(1): 70-74 |
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| 作者姓名： | 张素琴 周建华 胡蓉 |
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| 作者单位： | 上海市第八人民医院中医科,上海,200235;松江区中心医院妇产科,上海,201600 |
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| 摘 要： | 目的观察淫羊藿苷对人胚肺二倍体成纤维细胞(MRC-5)衰老的干预作用与机制,为明确淫羊藿苷作为有效干预衰老的候选中药单体提供实验依据。方法 MRC-5进行体外培养,利用淫羊藿苷进行干预,观察细胞的传代次数;利用β-半乳糖苷酶染色观察衰老细胞阳性率;MTT法检测细胞活力;分别使用单细胞凝胶电泳和流式细胞仪检测H2O2诱导细胞DNA损伤和凋亡变化。结果淫羊藿苷能够延缓MRC-5细胞的复制性衰老,促进细胞增殖,减轻H2O2诱导的细胞DNA损伤,促进受损细胞的凋亡。结论淫羊藿苷能够延缓二倍体细胞的复制性衰老,这可能与其能够减轻细胞DNA损伤有关。
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| 关 键 词： | 人胚肺二倍体成纤维细胞 细胞衰老 淫羊藿苷 DNA损伤 增殖 凋亡 |
Study on the effect of Icariin on Senescence of Human Embryonic Lung Diploid Fibroblasts |
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| Zhang Suqin,Zhou Jianhua,Hu Rong. Study on the effect of Icariin on Senescence of Human Embryonic Lung Diploid Fibroblasts[J]. Geriatrics & Health Care, 2019, 25(1): 70-74 |
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| Authors: | Zhang Suqin Zhou Jianhua Hu Rong |
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| Affiliation: | (Department of Traditional Chinese Medicine,Shanghai Eighth People's Hospital,Shanghai,200235,P.R.China;Department of Obstetrics and Gynecology,Songjiang District Central Hospital,Shanghai,201600,P.R.China) |
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| Abstract: | Zhang Suqin;Zhou Jianhua;Hu Rong(Department of Traditional Chinese Medicine,Shanghai Eighth People's Hospital,Shanghai,200235,P.R.China;Department of Obstetrics and Gynecology,Songjiang District Central Hospital,Shanghai,201600,P.R.China) |
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| Keywords: | Human embryonic lung diploid fibroblasts Cellular senescence Icariin DNA damage Proliferation apoptosis |
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