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新生儿血型检测的结果分析
引用本文:蔡旭清,孙爱农,孙雯婷.新生儿血型检测的结果分析[J].国际检验医学杂志,2014,0(11):1474-1475.
作者姓名:蔡旭清  孙爱农  孙雯婷
作者单位:蔡旭清 (广东省中山火炬开发区医院,广东中山,528437); 孙爱农 (中山市中心血站,广东中山,528403); 孙雯婷(中山市石岐区安监分局,广东中山,528400);
基金项目:中山市医学科研基金资助项目(2013A020159).
摘    要:目的:了解新生儿 ABO 正反定型符合率,研究提高新生儿血型测定准确性的方法。方法用传统试管法检测当地新生儿 ABO 血型,以微柱凝胶卡和改良试管法、增强剂法及常规试管法、纸片法、微板法、聚凝胺法等7种方法检测抗-A、抗-B、抗-D 抗体的效价,并进行新生儿血型检测比较。结果新生儿 ABO 正反定型符合率为83%(913/1100);检测15份抗-A、抗-B、抗-D 抗体的效价,敏感性最高是改良试管法和增强剂法,最差的是纸片法,新生儿标本检测结果类似。结论ABO 正反定型应增加血浆(血清)量并延长孵育时间或选用增强剂,从而提高新生儿血型检测的准确性。

关 键 词:新生儿  脐带血  ABO  血型  Rh(D)血型  正反定型

Analysis of neonatal blood testing
Cai Xuqing,Sun Ainong,Sun Wenting.Analysis of neonatal blood testing[J].International Journal of Laboratory Medicine,2014,0(11):1474-1475.
Authors:Cai Xuqing  Sun Ainong  Sun Wenting
Institution:1. Zhongshan Torch Development Zone Hospital, Zhongshan, Guangdong 528437, China 2. Blood Center of Zhongshan City, Zhongshan, Guangdong 528403 3. Administration of Work Safety of Shiqi District, Zhongshan, Guangdong 528400, China)
Abstract:Objective To understand the neonatal ABO type positive and reverse typing consistent rate and to improve the accuracy of the methods of neonatal blood.Methods The traditional tube method was used to detect the local neonatal ABO blood.The method of micro gel cards,improved tube method,enhancer and traditional tube method,paper,microplate,polybrene method to detect anti A,anti B,anti D titer,and neonatal blood detection comparator.Results Neonatal ABO positive and negative stereo types compliance rate was 83% (913/1 100);Detecting 15 copies of anti A,B,D titer,the highest sensitivity was improved intensifier tube method and the worst is the method of paper.The method of neonatal specimens was with the similar results.Conclusion ABO positive and negative stereo types should be increased by plasma (serum)levels and extending the incubation time,or using the"enhancer"of this study,thereby improving the accuracy of the newborn blood testing.
Keywords:newborns  umbilical cord blood  ABO blood type  Rh (D) blood type  positive and reverse typing
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