Determination of the molecular size of the 5-HT3 receptor binding site by radiation inactivation |
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| Authors: | H Gozlan L E Schechter F Bolanos M B Emerit M C Miquel M Nielsen M Hamon |
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| Institution: | INSERM U288, Neurobiologie Cellulaire et Fonctionnelle, Faculté de Médecine, Pitié-Salpêtrière, Paris, France. |
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| Abstract: | The radiation inactivation technique has been used to estimate the molecular size of the 5-HT3 receptor binding site labelled by 3H]zacopride, in comparison with that of the 5-HT1A receptor binding site labelled by 3H]8-OH-DPAT, in rat cortical membranes. The calculated molecular weight of the 5-HT3 site: 35.4 +/- 2.2 kDa (mean +/- S.E.M., n = 4) was significantly less than that of the 5-HT1A site: 62.9 +/- 1.8 kDa (mean +/- S.E.M., n = 4) and of other 5-HT1 and 5-HT2 receptors of the G-protein coupled family. These data further support that the 5-HT3 receptor is not coupled to G-proteins in the rat brain. |
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