再生障碍性贫血患者血清IL-10、IL-12和sFas水平及其意义

目的探讨再生障碍性贫血(再障)患者血清白细胞介素10(IL-10)、白细胞介素12(IL-12)和可溶性Fas(sFas)水平及其临床意义。方法利用双抗体夹心酶联免疫吸附法(ELISA)检测30例再障患者和15例骨髓增生异常综合征(MDS)患者血清IL-10、IL-12和sFas水平。结果与正常组比较,重型再障(SAA)患者血清IL-10、IL-12和sFas水平均明显升高(P均<0.01);慢性再障(CAA)和MDS患者血清IL-10和sFas水平均明显升高(P均<0.01),而IL-12水平较正常有上升的趋势,但无统计学意义(P>0.05);SAA和CAA患者血清IL-10、IL-12水平较MDS患者有上升的趋势,但组间无统计学意义(P均>0.05);SAA和MDS患者血清sFas水平较CAA患者明显升高(P<0.05,P<0.01),而MDS患者较SAA患者血清sFas水平有上升的趋势,但无显著性差异(P>0.05)。结论血清高水平的IL-10、IL-12和sFas可能参与再障骨髓造血干/祖细胞衰竭的病理生理过程;检测上述细胞因子水平,为再障患者监测病情、评价疗效、指导临床治疗及预后判断提供较为客观的指标。

Significance of Serum Levels of IL- 10,IL- 12 and sFas in Patients with Aplastic Anemia

Objective To study the levels of interleukin 10(IL 10), interleukin 12(IL 12) and sFas in patients with aplastic anemia(AA) and their clinical significance. Method Enzyme linked immunosorbent assay(ELISA) was used to detect IL 10, IL 12 and sFas levels in serum of 30 cases with AA and 15 cases with myelodysplastic syndromes(MDS). Results The higher serum levels of IL 10, IL 12 and sFas were observed in patients with SAA than in controls(P< 0 01). The IL 10 and sFas levels in patients with chronic aplastic anemia(CAA) and MDS were significantly higher than those in controls(P< 0.01). The IL 12 levels tended to increase, but there was no significant difference between CAA, MDS patients and controls(P >0.05). Further more,IL 10 and IL 12 levels in patients with SAA and CAA were higher than in those with MDS, but there was no significant difference(P >0.05). Serum levels of sFas in patients with SAA and MDS were significantly higher than those with CAA(P< 0.05 and P< 0.01).Although serum levels of sFas in patients with MDS were higher than those with SAA, but was not significant(P >0.05). Conclusions Higher serum levels of IL 10, IL 12 and sFas may be involved in the destruction of marrow hematopoietic cells in AA. They may be valuable parameters in monitoring the disease progress and evaluating the curative effect and prognosis of AA.