| Losartan inhibited expression of matrix metalloproteinases in rat atherosclerotic lesions and angiotensin Ⅱ-stimulated macrophages |
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| 引用本文： | Liang C,Wu ZG,Ding J,Jiang JF,Huang GZ,Du RZ,Ge JB. Losartan inhibited expression of matrix metalloproteinases in rat atherosclerotic lesions and angiotensin Ⅱ-stimulated macrophages[J]. Acta pharmacologica Sinica, 2004, 25(11): 1426-1432 |
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| 作者姓名： | Liang C Wu ZG Ding J Jiang JF Huang GZ Du RZ Ge JB |
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| 作者单位： | Chun LIANG,Zong-gui WU,Jian DING,Jian-fei JIANG,Gao-zhong HUANG,Rong-zeng DU,Jun-bo GEDepartment of Cardiology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China; Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital,Fudan University,Shanghai 200032,China |
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| 基金项目： | Project supported by the Major State Basic Research Develop-ment Programof China(G2000056903). |
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| 摘 要： | AIM: To explore whether the angiotensin Ⅱ (Ang Ⅱ) receptor 1 (AT1) antagonist, losartan could reduce activity and expression of matrix metalloproteinases (MMPs) in rat atherosclerotic plaques. METHODS: Male Wistar-Kyoto rats were ip injected a single dose of vitamin D3 600 kU·kg-1·month-1 and fed an atherogenic diet for 4 months to induce experimental atheroma. Then either placebo or losartan 50 mg·kg-1·d-1 was administered in rats for another 2 months. In vitro, the effect of losartan 0.1-10μmol/L on the expression of MMP-2 and MMP-9 was investigated in Ang II-stimulated rat peritoneal macrophages. The expression and activity of MMP-2 and MMP-9 were monitored by Western blot, RT-PCR, and SDS-PAGE zymography analysis. RESULTS: High levels of MMP-2 and MMP-9 were expressed in rat atherosclerotic lesions. Losartan significantly reduced the activity and expression of MMP-2 and MMP-9 compared with the placebo group (MMP-2, 5861±539 vs 8991±965, P<0.05; MMP-9, 10527±1002 vs 14623±2462, P<0.01). In
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Losartan inhibited expression of matrix metalloproteinases in rat atherosclerotic lesions and angiotensin II-stimulated macrophages |
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| Liang Chun,Wu Zong-gui,Ding Jian,Jiang Jian-fei,Huang Gao-zhong,Du Rong-zeng,Ge Jun-bo. Losartan inhibited expression of matrix metalloproteinases in rat atherosclerotic lesions and angiotensin II-stimulated macrophages[J]. Acta pharmacologica Sinica, 2004, 25(11): 1426-1432 |
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| Authors: | Liang Chun Wu Zong-gui Ding Jian Jiang Jian-fei Huang Gao-zhong Du Rong-zeng Ge Jun-bo |
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| Affiliation: | Chun LIANG,Zong-gui WU,Jian DING,Jian-fei JIANG,Gao-zhong HUANG,Rong-zeng DU,Jun-bo GEDepartment of Cardiology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China, Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital,Fudan University,Shanghai 200032,China |
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| Abstract: | |
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| Keywords: | MMP-2 MMP-9 PD RT-PCR SDS-PAGE ATI |
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