流式细胞分析梅毒患者外周血T淋巴细胞的免疫表型

目的　研究人类对梅毒螺旋体的细胞免疫反应特征 ,分析进入血液后的梅毒螺旋体对机体外周血中各淋巴细胞亚群及自然杀伤细胞 (NK)的影响情况。方法　通过流式细胞仪 ,运用标有异硫氰酸荧光素 (FITC)或藻红素 (PE)的CD单抗 ,分析了 2 4例未经驱梅治疗过的早期梅毒患者 (含泛发型皮疹、掌跖型皮疹及无皮疹型各 8例 )外周血中各T淋巴细胞免疫表型的特点。结果　 2 4例梅毒患者的外周血中总T、TH、NK细胞及TH/Ts的比值均明显低于正常人对照组 (P <0 0 1) ,而Ts的比例则显著高于正常人对照组 (P <0 0 1) ;另外皮疹呈泛发型、掌跖型、无皮疹型三组患者在上述免疫表型的比例方面相互比较 ,无统计学差异 (P >0 0 5 )。结论　未经驱梅治疗过的早期梅毒患者存在着明显的细胞免疫抑制 ,其中皮疹呈泛发型、掌跖型、无皮疹型三者基本上反映了进入血液后的梅毒螺旋体在不同皮肤屏障功能下的表现形式 ,而与机体外周血中T淋巴细胞亚群的变化之间无明显的相关关系

Analysis of Peripheral Blood Lymphocyte Immunophenotypes in Syphilitic Patients with Flow Cytometry

Objective To characterize the human cell mediated immune response to Treponema pallidum(TP), and to determine the effect of TP infection on the various lymphocyte subpopulations and natural killer cells in the peripheral blood of the patients. Methods Twenty four untreated patients with early syphilis were analyzed,including 3 groups of 8 patients with disseminated syphilides,palmoplantar syphilides and latent syphilis each. Monoclonal antibodies of CD labeled with either fluorescein isothiocyanate or phycoerythrin were used on a flow cytometry to detect the changing of peripheral blood lymphocyte immunophenotypes. Results The results showed that the percentages of panT,T H,NK cells and the ratio of T H/T S were significantly lower (P<0.01) and those of T S cells were significantly higher (P<0.01) than those of normal controls. No statistical difference of the immunophenotype distribution was found among groups of disseminated lesions, palmoplantar syphilides, and latent syphilis (P>0.05). Conclusion It suggests that there is prominent suppression of cellular immunity in the untreated patients suffering from early syphilis. Meanwhile, the difference of 3 groups in skin lesions was due to the different dermatobarrier function against the systemic invasion of TP, rather than attributed to the alteration of the lymphocyte immunophenotypes in the peripheral blood.