| 牵张负荷对大鼠膀胱Cajal间质细胞超微形态及c-kit基因表达影响 |
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| 引用本文： | 路扬,李龙坤,王永权.牵张负荷对大鼠膀胱Cajal间质细胞超微形态及c-kit基因表达影响[J].第三军医大学学报,2009,31(13). |
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| 作者姓名： | 路扬 李龙坤 王永权 |
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| 作者单位： | 第三军医大学西南医院全军泌尿外科研究所,重庆,400038 |
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| 摘 要： | 目的 通过研究膀胱Cajal间质细胞(Interstitial cells of cajal,ICCs)超微形态及c-kit基因水平变化,阐明牵张负荷对膀胱Cajal细胞兴奋性的调控作用.方法 结扎膀胱颈,构建大鼠膀胱出口部分梗阻致逼尿肌过度活动模型,通过扫描电镜方法观察牵张负荷条件下逼尿肌过度活动组与正常对照组膀胱ICCs细胞超微形态变化,观察牵张负荷条件下逼尿肌稳定组和逼尿肌过度活动组与正常对照组膀胱ICCs细胞酪氨酸激酶受体(c-kit)在基因水平表达差异.结果 逼尿肌过度活动组ICCs细胞形态和c-kit基因水平表达最较正常对照组有明显改变(P＜0.01).结论 牵张负荷对ICCs细胞有调控作用,可能的作用机制在于改变ICCs细胞形态及上调c-kit表达.
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| 关 键 词： | 牵张负荷 ICCs细胞 |
Effects of stretch load on the ultramicrostructure and c-kit gene expression of the bladder ICCs in rats |
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| LU Yang,LI Long-kun,WANG Yong-quan.Effects of stretch load on the ultramicrostructure and c-kit gene expression of the bladder ICCs in rats[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(13). |
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| Authors: | LU Yang LI Long-kun WANG Yong-quan |
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| Institution: | LU Yang,LI Long-kun,WANG Yong-quan (Urinary Surgery Research Institute,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To investigate the effects of stretch load on the ultramicrostructure and c-kit expression of the bladder interstitial cells of cajal (ICCs) in rats. Methods Rat model with detrusor overactivity due to partial outflow obstruction with bladder neck ligation were established. The effects of stretch load on the changes of ultramicrostructure of the bladder ICCs were observed by scanning electron microscopy. The changes in c-kit gene expression of ICCs in stable, overactive, and normal detrusors were ... |
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| Keywords: | c-kit |
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