兔髓核细胞诱导大鼠骨髓间充质干细胞分化为髓核细胞的研究

目的 研究体外新西兰大白兔髓核细胞(nucleus pulposus cells)与SD大鼠骨髓间充质干细胞(mes-enchymal stem cells,MSCs)共培养时,兔髓核细胞与大鼠MSCs直接和间接接触对MSCs分化为髓核细胞的影响.方法 DAPI (4‘,6-二咪基-2‘-苯吲哚盐酸)标记原代髓核细胞后,分别与第三代MSCs按接触组和非接触组(Transwell培养系统)共培养.每隔24小时应用免疫荧光观察MSCs的形态学变化,并用RT-PCR方法检测Ⅱ型胶原和可凝集蛋白多糖(Aggrecan)的表达.结果 直接接触培养组中可见分化的MSCs形态和功能接近髓核细胞;非直接接触组的MSCs未见变化.结论 髓核细胞与MSCs的直接接触,是诱导MSCs分化为髓核细胞的重要因素.

Study on differentiation of rat mesenchymal stem cells promoted by co-cultured nucleus pulposus cells of rabbit

Objective To explore the related mechanisms of rat mesenchymal stem cells (MSCs) of rat differ-entiating into nucleus pulposus cell in a simulated intervertebral disc micro-environment . Methods The primarynucleus pulposus cell was isolated and purified, after labeled by DAPI, cultured with the 3-th generation ofMSCs through direct and indirect ( millicell TM-HA semipermeable membrane) contact. Each 24 hours later,cell immofluorescence was carried to discriminate two kinds of cells. RT-PCR was carried to detect the expressionof the Aggrecan and type II collagen. Results There expression of Aggrecan and Type II collagen could be de-tected in direct contact group, there was no expression in indirect contact group. Conclusion It demonstratesthat the direct contact of MSCs and nucleus pulposus cell is essential for MSCs differentiation into nucleus pulposuscell.