Detection of virulence genes in Malaysian <Emphasis Type="Italic">Shigella</Emphasis>species by multiplex PCR assay期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Detection of virulence genes in Malaysian <Emphasis Type="Italic">Shigella</Emphasis>species by multiplex PCR assay

Authors:	Email author" target="_blank">Kwai?Lin?Thong Email author Susan?Ling?Ling?Hoe SD?Puthucheary Rohani?Md Yasin

Institution:	(1) Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;(2) Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;(3) Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia

Abstract:	Background In Malaysia, Shigella spp. was reported to be the third commonest bacterial agent responsible for childhood diarrhoea. Currently, isolation of the bacterium and confirmation of the disease by microbiological and biochemical methods remain as the "gold standard". This study aimed to detect the prevalence of four Shigella virulence genes present concurrently, in randomly selected Malaysian strains via a rapid multiplex PCR (mPCR) assay.

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