尼古丁对帕金森病大鼠纹状体多巴胺及黑质自由基含量的影响

目的 :研究尼古丁对帕金森病大鼠的影响 ,探讨其对 PD的作用机制。方法 :通过 6 - OHDA脑立体定向注射术建立大鼠帕金森病模型。采用生化方法观察不同剂量尼古丁对帕金森病大鼠的作用 ,检测黑质自由基、抗氧化剂及多巴胺含量的变化。结果 :造模前及造模后皮下注射尼古丁的 PD大鼠 ,黑质自由基及抗自由基酶及多巴胺含量较PD组有明显改善 (P<0 .0 5 )。结论 :尼古丁可减轻 6 - OHDA对黑质 DA能神经元的损伤 ,对 PD大鼠具有保护作用

Effects of nicotine on dopamine and free radical in 6-OHDA-indiced Parkinon's disease

Objective: To investigate the effects of nicotine on 6 OHDA induced degeneration of nigrostriatal dopaminergic neurons in rats. Methods: 6 OHDA was stereoscopically injected to right side of the mesencephic ventral tegmental area (VTA) and substantia nigra pars compacta (SNpc). Nicotine of different dosages was intraperitoneally injected two weeks before or after the operation. Using biochemical techniques, we observed the changes of the free radical and dopamine content in striatum and subatantia migra. Result: Chronic nicotine treatment given 2 weeks uefore 6 OHDA was injected into the brain, the free radical were decreased in substantia nigra and dopamine content in striatum were increased compared to the PD group ( P <0.05). Chronic nicotine treatment given 2 weeks after PD models were successful, the indexes of the group were chang ed compared to the PD group ( P <0.05). Conclusion: Nicotine can protect dopamine neurons of substantia nigra from the damage of 6 OHDA.