| 大鼠骨髓间充质干细胞的分离、培养和鉴定 |
| |
| 引用本文： | 倪玉霞,李澎,李贻奎,束云.大鼠骨髓间充质干细胞的分离、培养和鉴定[J].广西医科大学学报,2009,26(1). |
| |
| 作者姓名： | 倪玉霞 李澎 李贻奎 束云 |
| |
| 作者单位： | 1. 广西医科大学第一附属医院麻醉科,南宁,530021
2. 中国中医科学院西苑医院 |
| |
| 基金项目： | 国家重点基础研究发展规划(973计划) |
| |
| 摘 要： | 目的:培养符合实验要求的大鼠骨髓间充质干细胞(BMSCs)并进行鉴定,为进一步的研究打基础.方法:分别采用全骨髓贴壁培养法和密度梯度离心法培养大鼠骨髓间充质干细胞,对细胞的形态及生长特性进行观察,并应用流式细胞术对细胞表面抗原CD34、CD44、CD45、CD29、CD90以及CD11b进行表型鉴定.结果:两种方法培养的细胞均为成纤维样细胞,呈集落式生长,全骨髓培养法培养的BMSCs表达CD34-(95.4%),CD44+(94.2%),CD45-(91.6%),CD29+(93.8%),CD90+(98%),CD11b-(87.6%),梯度离心法培养的BMSCs表达CD34-(96.9%),CD44+(97.3%),CD45-(97.5%),CD29+(99.4%),CD90+(99.8%),CD11b-(96%).结论:两种方法均可分离培养出较纯化的BMSCs,但全骨髓贴壁培养法简单易行,原代培养周期较短.
|
| 关 键 词： | 骨髓间充质干细胞 流式细胞术 细胞培养 表型鉴定 |
THE ISOLATING,CULTURING AND IDENTITY OF RAT MARROW MESENCHYMAL STEM CELLS |
| |
| Ni Yuxia,Li Peng,Li Yikui,et al..THE ISOLATING,CULTURING AND IDENTITY OF RAT MARROW MESENCHYMAL STEM CELLS[J].Journal of Guangxi Medical University,2009,26(1). |
| |
| Authors: | Ni Yuxia Li Peng Li Yikui |
| |
| Institution: | Ni Yuxia,Li Peng,Li Yikui,et al.(the First Affiliated Hospital of Guangxi Medical Universerty,Nanning 530021 China) |
| |
| Abstract: | Objective:To culture rat marrow mesenchymal stem cells(BMSCs) fitting the requirement of experimentation for further study.Methods:Adopt whole bone marrow adherent cultivation or density gradient centrifugate cultivation to culture rat BMSCs,Observed the morphology and characteristics of cultured cells,identified the surface marker identification of CD34,CD44,CD45,CD29,CD90 and CD11b of cells by flow cytometry.Result:Cells cultured by whole bone marrow adherent cultivation showed CD34-(95.4%),CD44+(94.2%),C... |
| |
| Keywords: | mesenchymal stem cells flow cytometry cell culture phenotype identify |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
