| B(A)血型等位基因分型研究 |
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| 引用本文： | 刘长利,苗天红,刘亚庆,闫芳,刘素芳,刘凯. B(A)血型等位基因分型研究[J]. 中国输血杂志, 2012, 25(5): 429-431 |
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| 作者姓名： | 刘长利 苗天红 刘亚庆 闫芳 刘素芳 刘凯 |
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| 作者单位： | 北京市红十字血液中心,北京,100088 |
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| 基金项目： | 北京市科技新星计划(20061333) |
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| 摘 要： | 目的了解血清学定型为B(A)血型标本的分子基础。方法采用PCR-SSP方法检测以血清学方法定型为B(A)血型的4名献血者和8例送检患者标本的ABO基因型;对于有O等位基因的杂合子标本,使用特异性引物分别扩增O和B等位基因的7号外显子编码序列,并做测序分析。结果 12例采用血清学方法定为B(A)血型标本的ABO基因型分别为B/B型1例(8.33%),B/O1型4例(33.33%),B/O2型7例(58.33%);序列分析显示:B(A)02等位基因为5例(41.67%),B(A)04等位基因为7例(58.33%)。结论 B(A)04和B(A)02等位基因可能是我国北方汉族人群B(A)血型中主要的遗传类型。
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| 关 键 词： | ABO亚型 B(A)血型 基因分型 序列分析 等位基因 |
Molecular of on B(A) blood group in Chinese individuals |
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| LIU Changli , LIU Yaqi , YAN Fang , LIU Sufang , LIU Kai , MIAO Tianhong. Molecular of on B(A) blood group in Chinese individuals[J]. Chinese Journal of Blood Transfusion, 2012, 25(5): 429-431 |
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| Authors: | LIU Changli LIU Yaqi YAN Fang LIU Sufang LIU Kai MIAO Tianhong |
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| Affiliation: | ,Corresponding author: MIAO Tianhong.Beijing Red Cross Blood Center,Beijing 100088,China |
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| Abstract: | Objective To study the molecular background of B(A) blood group identified by serological tests.Methods PCR-SSP technique and DNA sequencing analysis were performed by using specially designed primers.Results One case of B/B,4 cases of B/O1 and 7 cases of B/O2 were found after genotyping on 12 unrelated B(A) phenotype samples.DNA sequencing analysis showed 7 cases of B(A)04 and 5 cases of B(A)02.Conclusion B(A)04 and B(A)02 should be a predominant alleles in Han population of Chinese northern. |
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| Keywords: | ABO subgroup B(A) blood group Genotype Sequencing analysis Alleles |
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