密蒙花总黄酮含药血浆对干眼症细胞模型Bax mRNA及Bcl-2 mRNA表达的影响

目的：观察密蒙花总黄酮含药血浆对干眼症细胞模型中泪腺上皮细胞Bax mRNA及Bcl-2 mRNA表达的影响。

方法：将培养生长状态良好的泪腺上皮细胞，随机分为无雄激素培养黄酮治疗组(以下简称密蒙花总黄酮组)，含雄激素培养对照组(以下简称雄激素组)，无雄激素培养空白组(以下简称空白组)。分别加入含密蒙花总黄酮血浆、丙酸睾酮、含空白血浆开始进行干预。建立干眼症细胞模型。用Trizol试剂提取细胞总RNA。RT-PCR法检测泪腺上皮细胞中Bax mRNA及Bcl-2 mRNA的表达。

结果：密蒙花组、雄激素组能够抑制泪腺上皮细胞中Bax mRNA的表达，与空白组比较差异有显著性(P<0.01)； 密蒙花组Bax mRNA的表达低于雄激素组，比较差异有显著性(P<0.01)。密蒙花组、雄激素组Bcl-2 mRNA的表达均高于空白组，比较差异有显著性(P<0.01)； 密蒙花组Bcl-2 mRNA的表达高于雄激素组，比较差异有显著性(P<0.01)。

结论：密蒙花总黄酮含药血浆作用于雄激素水平下降所致干眼症的细胞模型可抑制Bax mRNA的表达，使Bax mRNA的表达量降低，同时促进Bcl-2 mRNA的表达，使Bcl-2 mRNA的表达量增加。密蒙花总黄酮含药血浆很可能是通过抑制泪腺上皮细胞中Bax mRNA及促进Bcl-2 mRNA表达的途径而达到抑制细胞凋亡的作用。

Influence for Cell model of dry eye after intervention of plasma containing Buddleja Officinali Flavone and the expression of Bax mRNA and Bcl-2 mRNA in Lacrimal gland epithelial cells

AIM:To observe the influence of drug-containing plasma on the lacrimal gland epithelial cells and the expression of Bax mRNA and Bcl-2 mRNA in Lacrimal gland epithelial cells.

METHODS:Randomly divided the lacrimal gland epithelial cells in good condition into the flavonoid treatment group without androgen cultural(hereinafter referred to as Buddleia flavonoids group), the control group with androgen cultural(hereinafter referred to as androgen group), and the blank group without androgen cultural(hereinafter referred to as the blank group). Total flavonoids with Buddleia plasma, testosterone propionate and blank plasmain were added to begin the interposal. Then, cell models of dry eye syndrome were established. Trizol reagent was used to extract the whole cell RNA. RT-PCR method was used to detect the expression of the Bax mRNA and Bcl-2 mRNA in lacrimal gland epithelial cells, and the results were contrasted.

RESULTS:The Buddleja group and the androgen group inhibited the expression of Bax mRNA in lacrimal gland epithelial cells, compared with the blank group the difference was significant(P <0.01), the expression of Bax mRNA in the Buddleia group is lower than that in the androgen group, the difference was significant(P <0.01), however, the expression of Bcl-2 mRNA in the Buddleia group and the androgen group were higher than that in the blank group, the difference was significant(P <0.01). The expression of Bcl-2 mRNA in Buddleia group is higher than the androgen group, the difference was significant(P <0.01).

CONCLUSION:Buddleja role of flavonoids in the drug- containing plasma androgen levels decrease in cell models of dry eye syndrome can inhibit the expression of Bax mRNA, decrease the amount of the expression of Bax mRNA, and promote the expression of Bcl-2 mRNA, increase the expression of Bcl-2 mRNA. It is possible that the Buddleja flavonoids drug-containing plasma achieve the inhibition of apoptosis by inhibiting the expression of the Bax mRNA and promoting that of Bcl-2 mRNA in lacrimal gland epithelial cells.