| 大骨节病区FA对软骨细胞ALPase活性的影响 |
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| 引用本文： | 郭平 徐曦. 大骨节病区FA对软骨细胞ALPase活性的影响[J]. 地方病通报, 1997, 12(1): 4-7 |
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| 作者姓名： | 郭平 徐曦 |
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| 作者单位： | 华北煤炭医学院，北京医科大学药学院!北京市，100083，北京医科大学药学院!北京市，100083，北京医科大学药学院!北京市，100083 |
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| 基金项目： | 国家“八五”科技攻关项目!85－917－01004 |
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| 摘 要： | 为探明大骨节病病区黄腐酸（Ｆｕｌｖｉｃａｃｉｄ，ＦＡ）对软骨内成骨过程中矿化的影响，采用４－甲基伞形酮磷酸酯（４－ＭｅｔｈｙｌｕｍｂｅｌｌｉｆｅｒｙｌＰｈｏｓｐｈａｔｅ，４－ＭＵＰ）为碱性磷酸酶（ＡｌｋａｌｉｎｅＰｈｏｓｐｈａｔａｓｅ，ＡＬａｓｅ）作底物，并结合Ｇｏｍｏｒｉ染色法，研究了ＦＡ对体外培养的肥大软骨细胞及基质在矿化过程中ＡＬＰａｓｅ活性影响。发现与视黄酸（Ｒｅｔｉｎｏｉｃａｃｉｄ，ＲＡ
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| 关 键 词： | 黄腐酸 碱性磷酸酶 大骨节病 软骨细胞 |
The Effects of Fulvic Acid from Kashin-Beck Disease Area on the ALPase Activity of Chondrocytes |
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| Guo Ping, Xu Xi, Xu Shanjin, Wang Kui. The Effects of Fulvic Acid from Kashin-Beck Disease Area on the ALPase Activity of Chondrocytes[J]. Endemic Diseases Bulletin, 1997, 12(1): 4-7 |
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| Authors: | Guo Ping Xu Xi Xu Shanjin Wang Kui |
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| Abstract: | In order to explore the effects of fulvic acid (FA) from Kashin-Beck Disease (KBD) area on mincralization of bone formation occurs by a process of endochondral ossification, 4-Methylumbelliferyl Phosphate (4-MUP) was taken as the substrate of the alkaline phosphatase(ALPase) to determine the activity of the ALPase. At the same time the activity of ALPase was identified by Gomori staining. The study showed the effects of FA from KBD area on the activity of ALPase during mineralization in vitro of cultured hypertrophic chondrocytes and that the matrix is similar to that of retinoic acid. FA from KBD area can increase the ALPase activity (P 100mg/L),the ALPase activity declines. |
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| Keywords: | Fulvic acid: Alkaline phosphatase Kashin-Beck Disease Chondrocytes |
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