当归对氧化低密度脂蛋白所致血管内皮细胞损伤的保护作用

通过培养人脐静脉内皮细胞，观察当归对氧化低密度脂蛋白（ｏｘ ＬＤＬ）损伤的内皮细胞（ＥＣ）乳酸脱氢酶（ＬＤＨ）的漏出量及扫描电镜下ＥＣ形态结构的影响，并观察当归在体外对Ｃｕ２＋诱导的低密度脂蛋白（ＬＤＬ）氧化修饰的作用。结果显示：在作用２４ｈ后，ｏｘ ＬＤＬ组ＬＤＨ漏出明显增加，超微结构明显受损。当归＋ｏｘ ＬＤＬ组ＬＤＨ漏出显著减少，超微结构基本正常。在体外，当归能明显抑制Ｃｕ２＋介导的ＬＤＬ氧化，且随着当归浓度的增高显示抑制作用增强。结果表明：当归对ｏｘ ＬＤＬ所致血管ＥＣ损伤具有保护作用，其机制可能与当归抗氧化作用有关。

Protective Effects of Angelica on Oxidized Low-density Lipoprotein Induced Damages of Vascular Endothelial Cells

Effects of angelica on cultured human umbilical vein endotheial cells(HUVEC)damaged by oxidized low density lipoprotein(ox LDL)and its mechanism were investigated.When EC were incubated with ox LDL for 24 hours,the scanning electron microscopic study showed that the cellular outline changed,the intercellular space enlarged,the cellualr membrane ruptured,and the lactate dehydrogenase(LDH)was markedly increased.Treatment with angelica at concentrations of 2 and 20 mg/ml caused a reduction in LDH,and the morphologically damaged HUVEC were alleviated.This study also investigated the effects of angelica on the LDL peroxidation by copper in vitro.The results showed inhibitory effects of angelica.These results suggested that angelica provided protection for HUVEC damaged by ox LDL,and that the protective effects of angelica might be due to anti lipid peroxidation via free radicaed scavenging activity.