Three-Dimensional Registration of Magnetic Resonance Image Data to Histological Sections with Model-Based Evaluation |
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Authors: | Michael S Breen Roee S Lazebnik David L Wilson |
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Institution: | (1) Department of Biomedical Engineering, Case Western Reserve University, Wickenden Building, 10900 Euclid Avenue, Cleveland, OH, 44106;(2) School of Medicine, Case Western Reserve University, Cleveland, OH;(3) Department of Radiology, University Hospitals of Cleveland & Case Western Reserve University, Cleveland, OH |
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Abstract: | We developed a three-dimensional (3D) registration method to align medical scanner data with histological sections. After
acquiring 3D medical scanner images, we sliced and photographed the tissue using, a custom apparatus, to obtain a volume of
tissue section images. Histological samples from the sections were digitized using a video microscopy system. We aligned the
histology and medical images to the reference tissue images using our 3D registration method. We applied the method to correlate
in vivo magnetic resonance (MR) and histological measurements for radio-frequency thermal ablation lesions in rabbit thighs. For
registration evaluation, we used an ellipsoid model to describe the lesion surfaces. The model surface closely fit the inner
(M1) and outer (M2) boundaries of the hyperintense region in MR lesion images, and the boundary of necrosis (H1) in registered
histology images. We used the distance between the model surfaces to indicate the 3D registration error. For four experiments,
we measured a registration accuracy of 0.96± 0.13 mm (mean±SD) from the absolute distance between the M2 and H1 model surfaces,
which compares favorably to the 0.70 mm in-plane MR voxel dimension. This suggests that our registration method provides sufficient
spatial correspondence to correlate 3D medical scanner and histology data. |
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Keywords: | Image registration Histology Tissue typing with medical images Interventional magnetic resonance imaging (iMRI) |
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