蜜蜂快速衰老模型的建立

目的 探讨把蜜蜂作为一种实验动物,建立一种新的快速衰老动物模型的方法.方法 通过适当高温、缺氧结合震动环境,建立蜜蜂快速衰老动物模型,并通过观察蜜蜂的外观变化及测量蜜蜂整体匀浆中总SOD活力和GSH-PX活力的变化情况,结合病理切片确定合适的造模条件.结果 适当的高温、缺氧加噪音可使蜜峰出现衰老指证.蜜蜂匀浆中SOD活力、GSH-PX活力明显降低;脑组织和肠组织出现明显病理变化,但未达到最严重的程度,有利于药物对模型的纠正.结论 :在35℃、缺氧及噪音环境中4 h,可造成蜜峰衰老模型.

Establishment of Rapid Aging Bee Model

Objective To investigate the possibility of taking bees as laboratory animals and establishing the new rapid ag-ing animal model. Methods The bee model was established under environment of high temperature, lack of oxygen, and vibration. The appropriate conditions for establishing the model were investigated by observing the changes of appearance and measuring the activities of the total SOD and GSH-PX in the whole homogenate of bees. Meanwhile, the changes of biopsy were observed. Results Bees aging model could been set up at the conditions of appropriate temperature, hypoxia and noise. The model has obvious indications of aging, such as reduced activity of SOD and GSH-PX, and obviously pathological changes of brain organization and intestinal tissue. The aging indications are not the most seriously and are suit-able for the studies on drug improving the aging indications. Conclusion Being in the enviroment of high temperature (35 ℃), hypoxia and noise for4 hours, can result in the better bee aging model.