东宝肝泰对大鼠高脂饮食脂肪性肝炎的预防作用

目的：探讨东宝肝泰对大鼠高脂饮食脂肪性肝炎的预防作用。方法：雄性SD大鼠24只，随机分为3组。正常组予标准饮食喂养；对照组予高脂饮食(普通饲料加20g／kg胆固醇加100g／kg猪油)喂养；预防组在高脂饮食喂养8周后加用东宝肝泰，均于第16周处死。测量大鼠体重、肝湿重、肝指数(肝湿重／体重)及脂肪重量，并测定血清脂质、肝功能和肝匀浆脂肪含量，通过HE染色观察肝细胞脂肪变性、炎症程度。结果：预防组和对照组大鼠均出现肥胖、高脂血症及血清转氨酶升高。与对照组相比，预防组各项指标均有改善趋势，但除血清天门冬酸氨基转移酶(AST)、总胆固醇(TC)外两组间比较并无统计学差异。结论：东宝肝泰对高脂饮食诱发的大鼠高脂血症性脂肪性肝炎可能无预防作用。

Prevention of dongbaogantai on nonalcoholic steatohepatitis in rats fed with a fat - rich diet.

Objective: To explore the role of dongbaogantai in the prevention of nonalcoholic steatohepatitis(NASH) induced by fat - rich diet. Methods: 24 SD rats were divided into normal group, control group and prevention group. Rats in control group were fed a fat - rich diet consisted of fat - rich diet, 100g/kg lard oil and 20g/kg cholesterol. Rats in prevention group were added dongbaogantai after eight weeks of fat - rich diet. Rats were sacrificed at the 16 week, then weight of rats, liver and fat were measured, serum and hepatic levels of lipid, serum aminotransferase were gained and the degrees of hepatic steatosis and inflammation were observed with H - E. Results: All model rats developed obesity, hyperlipidemia and serum aminotransferase increased. Compared with normal group, each index in prevention group and control group was significantly increased. And compared with control group, serum totalcholesterol (TC) and asparaminotransferase(AST) were improved significantly, other indexes showed no difference.Conclusion: It is possible that dongbaogantai has no prevention on nonalcoholic steatohepatitis in rats fed with a fat - rich diet.