富氢盐水对延迟复苏烫伤大鼠血压及肺组织抗氧化能力的影响

目的 观察富氢盐水对延迟复苏烫伤大鼠血压和肺组织抗氧化能力的影响.方法 制备富氢盐水(即氢气溶解度达到饱和的生理盐水,氢浓度为0.6 mmol/L).采用随机数字表法将20只SD大鼠分为富氢盐水组和生理盐水组,每组10只.2组大鼠背部致30%TBSAⅢ度烫伤,伤后7、9、17 h,分别经腹腔给予相当于总补液量体积1/2、1/4、1/4的富氢盐水或者生理盐水,补液总量按照4 mL·kg-1·%TBSA-1(Parkland公式)计算.观察实验过程中大鼠总体情况;伤后6、24 h测收缩压;伤后24 h取大鼠肺组织,检测S0D抑制率和丙二醛含量.对实验结果进行t检验.结果 2组大鼠实验过程中无一只死亡.富氢盐水组和生理盐水组伤后6 h的收缩压分别为(87±4)、(86±5)mm Hg(1 mm Hg=0.133 kPa),2组水平接近(t=0.213,P=0.834);伤后24 h,富氢盐水组收缩压(124±7)mm Hg]高于生理盐水组(115±6)mm Hg,t=2.958,P=0.008].富氢盐水组肺组织SOD抑制率为(0.465±0.014)%,高于生理盐水组(0.358±0.021)%,t=11.767,P=0.000].富氢盐水组的肺组织丙二醛含量(922±196)pmol/mg]低于生理盐水组(1118±212)pmol/mg,t=-2.142,P=0.046].结论 用富氢盐水对烫伤大鼠行延迟复苏,更有助于其血压恢复,并通过增强抗氧化酶的作用,减轻再灌注引起的肺组织细胞损伤.

Effect of hydrogen-rich saline on blood pressure and antioxidant ability of lung tissue in scalded rats following delayed resuscitation

Objective To study the effect of hydrogen-rich saline on blood pressure and antioxidant ability of lung tissue in scalded rats following delayed resuscitation. Methods The hydrogen-rich saline was prepared ( hydrogen-saturated normal saline with hydrogen concentration of 0.6 mmol/L). Twenty SD rats were divided into hydrogen-rich saline group (HS) and normal saline group (NS) according to the random number table, with 10 rats in each group. All the rats were subjected to 30% total body surface area (TBSA) full-thickness scald. Rats in HS and NS groups were infused with hydrogen-rich saline or normal saline with one half of the total fluid replacement volume as calculated according to the Parkland formula (4 spectively. The general condition of rats during the experiment was observed. The systolic pressure of rats was measured at PSH 6 and 24. All rats were sacrificed at PSH 24 to collect lung tissue for detecting superoxide dismutase (SOD) inhibition ratio and malondialdehyde (MDA) level. Data were processed with t test.Results All rats survived through the experiment. The systolic pressure of rats in HS group and NS group was respectively (87 ±4) mm Hg (1 mm Hg=0. 133 kPa) and (86 ±5) mm Hg at PSH 6, and the values were close ( t = 0.213, P = 0. 834); however the systolic pressure at 24 h was higher in HS group than in NS group (124±7) mmHgvs. (115 ±6) mmHg, t =2.958,P =0.008]. SOD inhibition ratio of lung tissue in HS group (0.465 ±0.014)%] was higher than that in NS group (0.358 ±0.021)%, t =11. 767, P =0. 000]. MDA level of lung tissue in HS group (922 ± 196) pmol/mg] was lower than that in NS group (1118 ±212) pmol/mg, t = -2. 142, P =0.046]. Conclusions Delayed resuscitation for scalded rats with hydrogen-rich saline is helpful in the recovery of systolic pressure, and it can ameliorate lung tissue injury caused by reperfusion through enhancing the effect of antioxidase.