舒芬太尼预处理对心肌缺血再灌注损伤的影响

目的观察缺血预处理及舒芬太尼预处理对大鼠心肌缺血再灌注的影响。方法建立48只大鼠心肌缺血再灌注损伤模型。随机分为假手术组（sham组）、对照组（CON组）、缺血预处理（IPC组）和舒芬太尼预处理（SPC组）各12例,于缺血前30min、缺血30min、再灌注90min时记录心电图、心率（HR）、平均动脉血压（MAP）及计算动脉压与HR乘积（RPP）。于实验结束前抽取动脉血进行CK-MB、LDH检测。遂后取出大鼠心脏,制作病理切片,缺血危险区（AAR）为红色,梗死区（IS）为白色,分别称重。结果与CON组比较,IPC组、SPC组MAP、RPP升高,心肌梗死面积及CK-MB、LDH降低（P〈0.05,P〈0.01）;与sham组比较,CON组MAP、RPP降低,CK-MB、LDH升高（P〈0.01）。结论舒芬太尼可模拟心脏缺血预处理作用,对心肌缺血再灌注损伤具有保护作用。

Effects of preconditioning on myocardial ischemic reperfusion injury of rats

Objective To study the effect of ischemic preconditioning and sufentanil precondition on myocardium against ischemia/reperfusion(I/R) injury.Methods The myocardial ischemia and reperfusion injury model was built.The animals were randomly divided into 4 groups: sham group(n=12);control group(CON,n=12);ischemic preconditioning group(IPC,n=12) and sufentanil preconditioning group(SPC,n=12).In control group the hearts were subjected to 30 min ischemia followed by 90 min reperfusion(I/R).In IPC group the hearts ...