Anucleate cell blue assay: a useful tool for identifying novel type II topoisomerase inhibitors |
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| Authors: | Oyamada Yoshihiro Ito Hideaki Fujimoto-Nakamura Mika Tanitame Akihiko Iwai Noritaka Nagai Kazuo Yamagishi Jun-Ichi Wachi Masaaki |
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| Affiliation: | Pharmacology & Microbiology Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Enoki 33-94, Suita, Osaka 564-0053, Japan. |
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| Abstract: | About 95,000 compounds were screened by the anucleate cell blue assay. Fifty-one of the hit compounds had various structures and showed inhibitory activity against DNA gyrase and/or topoisomerase IV. Moreover, the compounds exhibited antibacterial activity against a fluoroquinolone- and novobiocin-resistant strain of Staphylococcus aureus. The anucleate cell blue assay is therefore a useful tool for finding novel type II topoisomerase inhibitors. |
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