氯胺酮诱导的热休克蛋白70在大鼠脑组织中不同区域的表达

目的 研究氯胺酮诱导的热休克蛋白70(HSP70)在大鼠脑组织中不同区域的表达.方法 SD大鼠20只,随机均分为氯胺酮组(K组)和生理盐水组(N组),分别在腹腔注射氯胺酮80mg/kg和生理盐水3ml.24h后取鼠脑,应用HSP70单克隆抗体免疫组化染色检测胼胝体压部后皮质、海马、大鼠后扣带回和枕叶皮质中HSP70的表达,并用MIAS-2000图文分析系统分析上述区域HSP70阳性细胞百分率、阳性细胞密度(个/mm2)和阳性产物灰度值.结果 氯胺酮可诱导大鼠大脑特定区域表达HSP70;大脑各区域HSP70表达不同.结论 氯胺酮诱导HSP70在大脑特定区域的表达,提示可能产生神经细胞损害;氯胺酮对大脑特定区域产生损害.

Expression of HSP70 induced by ketamine in different regions of rat brain.

Objective To determine the heat shock protein 70(HSP70) expression induced by ketamine in different regions of rat brain.Methods Twenty rats were randomly divided into two groups with ten rats each.Rats in group K were injected intraperitoneally with ketamine 80 mg/kg,and those in group N with normal saline as the controls.Twenty-four hours later,the rat brain was removed,and HSP70 mono-clone antibody immunocytochemistry was performed to detect HSP70 expression in different regions of the rat brain.MIAS-2000 photography analytic software was used to analyze HSP70 expression.Results Ketamine induced HSP70 expression in rat brain,and HSP70 expression was different significantly in different regions.Conclusion Ketamine induces the expression of HSP70 in specific regions of rat brain,suggesting that ketamine might lead to neurological injures in these regions.