特应性和非特应性哮喘患儿血清白细胞介素-10和血浆内皮素-1表达水平的检测及其临床意义

［摘 要］目的：观察特应性与非特应性哮喘患儿血清白细胞介素-10（IL-10）、血浆内皮素-1（ET-1）和IgE水平，探讨其临床意义。方法：60例哮喘患儿同时采用过敏原皮肤点刺试验和血清IgE测定确定特应性状态，并根据结果分为特应性哮喘组32例与非特应性哮喘组28例，30例正常健康儿作为对照组。采用放射免疫法检测哮喘患儿在发作期和缓解期及对照组儿童血浆ET-1和IgE的含量，应用ELISA双抗体夹心法检测各组血清IL-10的含量。结果：发作期哮喘患儿血清IL-10的表达明显低于对照组（P<0.01），ET-1水平明显高于对照组（P<0.05），发作期及缓解期哮喘患儿的IgE水平均明显高于对照组（P<0.01）。哮喘组发作期患儿ET-1和IgE均高于缓解期患儿，IL-10低于缓解期患儿(P<0.01)。特应性组发作期患儿IL-10水平较非特应性组降低（P<0.05）,特应性组发作期和缓解期患儿ET-1、IgE水平较非特应性组均增高（P<0.05）。支气管哮喘患儿IL-10的含量与ET-1及IgE分别呈明显负相关(r=－0.592，r=－0.894，P<0.05)，ET-1与IgE呈明显正相关(r=0.623，P<0.05)。结论：血清IL-10和ET-1可能参与了小儿哮喘的病理生理过程，且特应性哮喘患者较非特应性哮喘患者血清IL-10、ET-1和IgE水平改变程度更明显。

Detection of levels of serum interleukin-10 and plasma endothelin in children with or without atopic asthma and their clinical significances

Objective To observe the changes of the levels of serum interleukin-10(IL-10),plasma endothelin(ET-1) and immunoglobulin E(IgE) in the children with or without atopic asthma and to discuss their clinical significances.Methods 60 asthma children conducted the Allergen skin prick tests and serum IgE measurement to determine atopic status at the same time,and they were divided into atopic asthma group(n=32) and non-atopic asthma group(n=28) according to the results.30 normal healthy children were selected as control group.The expressions of ET-1 and IgE in the asthma children during attack period and remission phase and the children in control group were detected by radioimmunoassay and the levels of serum IL-10 were detected by the double antibody sandwich ELISA.Results The level of serum IL-10 in the asthma children in the acute attack period was lower than those in control group(P<0.01),and the level of ET-1 was higher obviously than that in control group(P<0.05);the levels of IgE in the asthma children in the acute attack period and remission phase in asthma groups were obviously higher than that in control group(P<0.01).The levels of ET-1 and IgE of the atopic asthma children in acute attack period were higher than those of the atopic asthma children in remission phase,and the level of IL-10 was lower(P<0.01).The levels of serum IL-10 in atopic group of the acute attack period was lower than that in non-atopic group(P<0.05),and the levels of the ET-1 and IgE of the patients in the acute attack period and remission phase in atopic group were higher than those in non-atopic group(P<0.05).The relationships between IL-10 level and ET-1 and IgE showed obviously negative correlations(r=-0.592,r=-0.894,P<0.05),and the relationship between ET-1 and IgE showed obviously positive correlation(r=0.623,P<0.05).Conclusion The levels of serum IL-10 and ET-1 perhaps take part in the pathologic and physiological process of the children’s asthma and the changes of the levels of IL-10,ET-1 and IgE in the children with atopic asthma are more obvious.