塞来昔布对人甲状腺髓样癌TT细胞中VEGF和MMP-9表达的抑制作用及其机制

目的：探讨环氧化酶2(COX-2)选择性抑制剂塞来昔布对人甲状腺髓样癌TT细胞中血管内皮生长因子(VEGF) mRNA及基质金属蛋白酶-9(MMP-9) mRNA表达的影响，探讨塞来昔布抗肿瘤的作用机制。方法：人甲状腺髓样癌TT细胞株分为不同浓度塞来昔布组和对照组，各塞来昔布组分别给予不同浓度的塞来昔布（20、40和80 μmol?L-1），对照组给予F12K培养液，作用72 h后，采用RT-PCR方法检测不同浓度塞来昔布作用于TT细胞后VEGF mRNA和MMP-9 mRNA表达的变化。结果： 20、40和80 μmol?L-1的塞来昔布对甲状腺癌TT细胞VEGF mRNA和MMP-9 mRNA的表达均有抑制作用。40和80 μmol?L-1塞来昔布组TT细胞中VEGF/GAPDH及MMP-9/GAPDH值与对照组比较差异有统计学意义（P<0.05），结论： 塞来昔布浓度高于40 μmol?L-1时可明显降低VEGF mRNA和MMP-9 mRNA的分泌，可能对肿瘤生长有一定抑制作用。

Inhibitory effects of celecoxib on expressions of VEGF and MMP-9 in human medullary thyroid TT cells and their mechanisms

Objective To discuss the effect of cyclooxegenase-2(COX-2) selective suppressor celecoxib on the expressions of vascular endothelial growth factor(VEGF) mRNA and matrix metalloproteinases-9(MMP-9) mRNA in human medullary thyroid TT cells,and to approach the anticancer mechanism of celecoxib.Methods The TT cells of human medullary thyroid carcinoma were divided into control group and different concertrations of celecoxib groups,and the cells in celecoxib groups were treated with different concentrations of celecoxib(20,40 and 80 μmol·L-1),the cells in control group were treated with F12K culture fluid.The expressions of VEGF mRNA and MMP-9 mRNA in each group were detected by RT-PCR 72 h after treatment.Results the RT-PCR results showed that the expressions of VEGF mRNA and MMP-9 mRNA in TT cells were decreased after treated with 20,40 and 80 μmol·L-1 celecoxib.There was significance difference of the VEGF/GAPDH and MMP-9/GAPDH of TT cells between 40,80 μmol·L-1 celecoxib groups and control group(P<0.05).Conclusion The secretion of VEGF mRNA and MMP-9 mRNA is decreased obviously when the concentration of celecoxib is above 40 μmol·L-1,which may inhibit the growth of tumor.