胡桃醌对白血病细胞增殖的抑制作用

目的: 探讨胡桃醌对白血病细胞增殖的抑制作用，阐明胡桃醌抗白血病的机制。方法: 体外培养人白血病K562、Jurkat、U937、U266及NB4细胞株，按不同处理因素分组：调零组，含有培养基、MTT和三联溶解液；空白对照组，含有各种人白血病细胞、药物溶解介质、培养液、MTT 和三联溶解液；实验组，分别采用不同浓度（终浓度为0.25、0.50、1.00、2.00、4.00、6.00、8.00和16.00 mg/L）的胡桃醌作用各种细胞株。MTT比色法检测胡桃醌对白血病细胞的增殖抑制作用。流式细胞术测定不同浓度胡桃醌对K562细胞株凋亡的影响。结果: 胡桃醌对白血病K562、Jurkat、U937、U266及NB4细胞株增殖均有较强的抑制作用，其半数抑制浓度(IC50)(48 h)分别为3.87、1.13、4.48、1.87和4.67 mg/L。其中胡桃醌对Jurkat和U266细胞的抑制效果最为明显（P<0.05），胡桃醌对其他3个白血病细胞株的IC50值均<40 mg/L，且胡桃醌对这5种细胞株增殖的抑制作用呈时间和剂量依赖性。流式细胞术检测显示，胡桃醌可诱导K562细胞凋亡，1、2、4、8和16 mg/L胡桃醌作用6 h，K562细胞凋亡率分别为(4.54±0.6) %、(11.5 4±0.6) %、(28.7±0.3) % 、(45.0±1.2) %和(76.1±1.4) %。结论: 胡桃醌可抑制多种白血病细胞株增殖，其机制可能与促进白血病细胞凋亡相关。

Inhibitory effect of juglone on proliferation of leukemia cells

Objective To investigate the inhibitory effect of juglone on the proliferation of leukemic cells and to clarify the mechanism of anti-leukemia of juglone.Methods The leukemic cell lines K562,Jurkat,U937,U266 and NB4 were cultured in vitro and divided into 4 groups as follows: zero group,containing medium,MTT and triple lysate;blank control group,containing a variety of human leukemia cells,drug dissolution media,culture medium,MTT and triple lysate;experiment groups,respectively using different concentrations(final concentration: 0.25,0.50,1.00,2.00,4.00,6.00,8.00,16.00 mg·L-1)of juglone disposal of various cell lines.The inhibitory effects of juglone of proliferation of leukemia cells were detected by MTT method.The apoptosis of K562 cells after treated with different concentrations of juglone was detected by flow cytometry(FCM).Results Juglone had strong inhibitory effects on the K562,Jurkat,U937,U266 and NB4 cell lines.Their IC50(48 h) were 3.87,1.13,4.48,1.87 and 4.67 mg·L-1,respectively,and the inhibitory effects on Jurkat and U266 cells(P<0.05) were most obvious.The IC50 values of juglone on other three leukemia cell lines(48 h) were less than 40 mg·L-1,and the inhibitory effects of juglone on these five cell lines presented dose-and time-dependent manner.FCM analysis showed that juglone could induce the apoptosis of K562 cells;the apoptotic rates of K562 cells were(4.54±0.6) %,(11.5 4±0.6) %,(28.7±0.3) %,(45.0±1.2) % and(76.1±1.4) %,respectively,after treated with 1,2,4,8 and 16 mg·L-1 juglone for 6 h.Conclusion Juglone can inhibit a variety of leukemia cell lines in vitro,and the mechanism may be related to promoting the apoptosis of K562 cells.