电纺纳米银/碳纳米纤维敷料的制备及抗菌效果评价

目的：利用静电纺丝技术制备纳米纤维敷料，并研究其抗菌效果。方法：采用聚丙烯腈(PAN)为前驱体高分子，利用静电纺丝技术及高温碳化方法制备碳纳米纤维敷料，并通过银镜反应制备纳米银/碳纳米纤维复合敷料。将2种样本与庆大霉素纸片及优拓（urgotul）敷料采用Kirby-Baucer法进行体外抑菌实验。结果：成功制备出具有纳米级支架结构且负载纳米银的的创面敷料。体外抑菌实验结果显示，纳米银/碳纳米纤维复合敷料和优拓对金黄色葡萄球菌、铜绿假单胞菌和大肠埃希菌标准菌株均有较好的抑菌效果；纳米银/碳纳米纤维复合敷料对于金黄色葡萄球菌及铜绿假单胞菌抑菌效果优于优拓，但对大肠杆菌的抑制效果低于优拓；碳纳米纤维未显示出具有抑菌能力。结论：纳米银/碳纳米纤维复合敷料具有纳米级支架结构，是具有较好抗菌能力的烧伤创面敷料。

Preparation of electrospun nanoscale silver / carbon nanofiber composite dressing and evaluation on its antibacterial efficacy

Objective To prepare nanofiber dressing by using electrospinning technology and determine its bacteriostatic efficacy.Methods Polyacrylonitrile(PAN) was used for precursor,by applying electrostatic spinning technology and high temperature carbonization method carbon nanofibers were prepared.Useing silver mirror reaction,nanoscale silver/carbon nanofiber composite dressing was prepared.According to Kirby-Baucer method,antibacterial extrasomatic test was conducted with these two samples and gentamicin scraps of paper and urgotul.Results The composite dressing had nanofiber scaffold structure and carried anoscale silver.The in vitro antibacterial experiment results showed that the nanoscale silver/carbon nanofiber composite dressing and urgotul had good antimicrobial effects on Staphylococcus aureus(Pae),E.coli(Eco) and Pseudomonas aeruginosa(Sau);the effects of composite dressing were better than urgotul on Pae and Sau,but was poor on Eco(P<0.05);the carbon nanofiber dressing had no antibacterial property.Conclusion The nanoscale silver / carbon nanofiber composite dressing with nanofiber scaffold structure and good antimicrobial effect is a good burn wound dressing.