党参皂苷D对HepG-2细胞增殖时间效应和细胞周期的影响

目的：观察党参皂苷D对HepG-2细胞增殖时间效应和细胞周期的影响，探讨轮叶党参抗癌活性成分及作用机制。方法：不同浓度（5、10、15、20和25 mg/L）党参皂苷D作用于体外培养的人肝癌HepG-2细胞，同时设空白对照组；采用 MTT法检测党参皂苷D对HepG-2细胞增殖抑制情况，流式细胞术检测细胞周期变化。结果：25 mg/L党参皂苷D作用12、24、48
和72 h后，HepG-2细胞增殖抑制率均明显高于对照组，差异有统计学意义(P<0.01)。党参皂苷D作用48、72、96及120 h后，各组细胞总数明显低于对照组，差异有统计学意义(P<0.05)；党参皂苷D 5、15、20及25 mg/L组间比较，差异有统计学意义(P<0.05)，且随着党参皂苷D浓度增加，HepG-2细胞增殖速度缓慢。细胞周期检测结果表明，党参皂苷D浓度为10、15、20和25 mg/L时，G0/G1期细胞所占百分比分别为55.97%±0.42%、57.16%±0.13%、57.48%±0.15%及60.39%±0.32%，与空白对照组（53.22%±0.28%）比较，差异均有统计学意义（P<0.05）；此浓度范围内S期细胞所占百分比分别为27.47%±1.42%、26.15%±2.71%、26.34%±0.67%及24.81%±0.46%，与空白对照组（33.47%±0.25%）比较，差异均有统计学意义（P<0.05）。党参皂苷D组的细胞周期发生了G0/G1期阻滞。结论：党参皂苷D在体外明显抑制HepG-2细胞生长，并能引起癌细胞DNA合成代谢紊乱。

Effects of lancemaside D on time effect of proliferation and cell cycle of HepG-2 cells

Objective To investigate the effect of lancemaside D on the time effects of cell proliferation and cell cycle of HepG-2 cells and explore the anticancer active ingredients and mechanisms of codonopsis lanceolata.Methods The HepG-2 cells were treated with different concentrations(5,10,15,20 and 25 mg·L-1) of lancemaside D in vitro,meanwhile control group was set up.The proliferation inhibition of HepG-2 cells was detected by MTT assay,and the change of cell cycle of HepG-2 cells was determined by flow cytometry.Results The results showed that the inhibitory rate of cell proliferation of HepG-2 cells were significantly higher than that in control group(P<0.01) after treated with 25 mg·L-1 lancemaside D for 12,24,48 and 72 h;the cell numbers in lancemaside D groups were significantly smaller than that in control group after treated with lancemaside D for 48,72,96 and 120 h(P<0.05),among 5,15,20 and 25 mg·L-1groups there were significant differences(all P<0.05);the HepG-2 cell proliferation speed was delated with the increasing of lancemaside D concentration.The cell cycle detection results showed that in the lancemaside D concentration range of 10-25 mg·L-1,the percentages of HepG-2 cells at G0/G1 phase were 55.97%±0.42%,57.16%±0.13%,57.48%±0.15% and 60.39%±0.32%,respectively,they were significantly higher than that in control group(53.22%±0.28%)(P<0.05).In the same concentration range of lancemaside D,the percentages of HepG-2 cells at S phage were 27.47%±1.42%,26.15%±2.71%,26.34%±0.67% and 24.81%±0.46%,they were significantly lower than that in control group(33.47%±0.25%)(P<0.05),which suggested a G0/G1 cell cycle arresting in lancemaside D group.Conclusion Lancemaside D strongly inhibits the growth of HepG-2 cells in vitro and furthermore cause cell DNA metabolism disturbance.