电离辐射对成纤维细胞中透明质酸蛋白及透明质酸酶1mRNA表达水平的影响

目的:观察电离辐射对成纤维细胞中透明质酸（HA）蛋白及透明质酸酶1（HAase1） mRNA表达水平的影响，并探讨其在放射性臂丛神经损伤发病机制中的作用。方法: 成纤维细胞分别采用0.5、1.0、2.0、4.0和6.0 Gy X射线照射，作为 5个剂量组，同时设假照射对照组（0 Gy）。透射电镜观察0、2.0、4.0和6.0 Gy X射线照射后48 h成纤维细胞的形态变化。采用ELISA法和RT-PCR法检测0、0.5、1.0、2.0、4.0、和6.0 Gy X射线照射后24及48 h成纤维细胞中HA蛋白及HAase1 mRNA表达水平的变化。结果：2.0 Gy X射线照射后48 h细胞染色质凝聚，有空泡形成；4.0 Gy X射线照射后48 h细胞核凹陷、染色质断裂；6.0 Gy X射线照射后细胞核凹陷、染色质固缩、出现空泡，部分胞浆溶解。不同剂量X射线照射后24 h HA蛋白表达均明显增高，与0 Gy组比较差异有统计学意义(P<0.05)；0.5～2.0 Gy X射线照射后48 h HA蛋白表达变化不明显,4.0和6.0 Gy组与 0 Gy组比较，差异有统计学意义（P<0.05）。0～6.0 Gy X射线照射后24 h HAase1 mRNA水平明显增高，与0 Gy组比较，差异有统计学意义（P<0.05）。1.0、4.0和6.0 Gy X射线照射后48 h HAase1 mRNA水平明显增高，与0 Gy组比较，差异有统计学意义(P<0.05)。结论：电离辐射可诱导人成纤维细胞中HA蛋白表达和HAase1 mRNA水平增高，可能对放射性臂丛神经损伤的发生发展起到一定的作用。

Effect of ionizing radiation on expression levels of hyaluronic acid protein and HAase1 mRNA in human fibroblasts

Objective To investigate the effect of ionizing radiation on the expression levels of hyaluronic acid(HA) protein and hyaluronidase(HAase1) mRNA in human fibroblasts and to discuss the role of HA protein and HAase1 mRNA in the pathogenic mechanism of radiation-induced brachial plexus injury.Methods The human fibroblasts were divided into 0.5,1.0,2.0,4.0 and 6.0 Gy X-rays radiation groups and sham irradiation group(0 Gy).The morphological changes of the fiber cells after irradiated by different doses of X-rays were observed.The expression levels of HA protein and HAase1 mRNA at 24 and 48 h after irradiated by different doses of 0,0.5,1.0,2.0,4.0 and 6.0 Gy X-rays were detected by ELISA and RT-PCR methods.Results The cells appeared vacuolization,and the nuclear chromatic began to get together after 2.0 Gy exposure;the nuclear saged and the chromatin fractured after 4.0 Gy exposure;the nuclear saged,chromatin fractured and the part of cell plasma was dissolved after 6.0 Gy exposure.Compared with 0 Gy group,the expressions of HA protein were increased significantly at 24 h after X-rays irradiation with the doses of 0.5,1.0,2.0,4.0 and 6.0 Gy(P<0.05) and the expression of HA protein had no significant change at 48 h after irradiation,but the expressions of HA protein in 4.0 and 6.0 Gy groups were increased significantly compared with 0 Gy group(P<0.05).The levels of HAase1 mRNA were markedly increased at 24 h after irradiated by different doses of X-rays(P<0.05).Compared with 0 Gy group,the expressions of HAase1 mRNA were increased significantly at 48 h after 1.0,4.0 and 6.0 Gy exposure compared with 0 Gy group(P<0.05).Conclusion The X-rays irradiation can induce the increase of the expression of HA protein and the level of HAase1 mRNA in human fibroblasts,and it may influence the occurrence and development of radiation-induced brachial plexus injury.