“肾阳虚证”下心肌梗死模型与单纯心肌梗死模型大鼠心肌形态学、心肌酶学及血液流变学的比较

目的：建立大鼠“肾阳虚证”下心肌梗死模型，探讨其与单纯心肌梗死模型大鼠在心肌形态学、心肌酶学及血液流变学方面的差异，为评价治疗胸痹心痛中药的药效学提供理论依据。方法：60只Wistar大鼠随机分为空白对照组、肾阳虚模型组、心肌梗死假手术组、单纯心肌梗死模型组及“肾阳虚证”下心肌梗死模型组，每组12只。在大鼠 “肾阳虚”情况下复制急性心肌梗死模型，测定各组大鼠心肌梗死面积 (MIS)，血清天门冬氨酸氨基转化酶（AST）、肌酸磷酸激酶（CK）及乳酸脱氢酶（LDH）活性，同时测定血小板黏附率（PAR）、血小板聚集率（PAG）、红细胞沉降率（ESR）、红细胞压积（HCT）、体外血栓长度、血栓干重与湿重以及血栓弹力图等参数。结果： 大鼠“肾阳虚证”下心肌梗死模型与单纯心肌梗死模型在MIS，血清AST、CK及LDH活性，PAR、PAG、ESR及HCT增加程度差异无统计学意义（P>0.05）；肾阳虚模型组、单纯心肌梗死模型组及“肾阳虚证”下心肌梗死模型组大鼠体外血栓干重及长度均明显增加（P<0.05或P<0.01），“肾阳虚证”下心肌梗死模型组的增加程度大于单纯心肌梗死模型组及肾阳虚模型组，但三者之间差异无统计学意义（P>0.05）；尽管“肾阳虚证”下心肌梗死模型组大鼠血栓弹力图r、k值的缩短程度及ma值的增大程度高于单纯心肌梗死模型组，但2组之间差异无统计学意义（P>0.05）。结论：大鼠“肾阳虚证”下心肌梗死模型与单纯心肌梗死模型心肌梗死面积、血清心肌酶学、红细胞压积、血沉、血小板功能、体外血栓重量及血栓弹力图等指标均无明显差异。

Comparison of myocardial morphology, myocardial enzymology and hemorheology between myocardial infarction models with kidney-yang deficiency and simple myocardial infarction models in rats

Objective To construct the myocardial infarction models in rats with kidney-yang deficiency and study the differences in myocardial morphology,myocardial enzymology and hemorheology between the myocardial infarction models with kidney-yang deficiency and the simple myocardial infarction models in rats and to provide evidence for evaluation of pharmacodynamics of medicine for curing chest and heart pain.Methods 60 Wistar rats were randomly divided into control group,kidney-yang deficiency models group,myocardial infarction sham operation group,myocardial infarction models group and myocardial infarction models with kidney-yang deficiency group,and there were 12 rats in each group.The myocardial infarction models under the status of the kidney-yang deficiency in rats were set up to determine the parameters of myocardial infarct size(MIS) and the activities of serum aspartate aminotransferase(AST),creatine phosphokinase(CK) and lactate dehydrogenase(LDH),at the same time,the platelet agglutinate rate(PAR),platelet aggregation(PAG),erythrocyte sedimentation rate(ESR),hematocrit(HCT) and the length,the dry weight,the wet weight of the thromb in vitro were tested,the parameters of thrombelastogram were also be detected.Results There were no obvious differences in MIS,the activities of CK,AST and LDH,and PAR,PAG,ESR and HCT between the myocardial infarction models with kidney-yang deficiency in rats and the simple myocardial infarction models(P>0.05);the dry weight of the thromb and the length of thromb in vitro of the myocardial infarction models with kidney-yang deficiency in rats were heavier than those of the simple myocardial infarction models and the kidney-yang deficiency models in rats,but there were no significant differences(P>0.05).The increasing extent of the values of r,k and the reducing extent of the value of ma in the myocardial infarction models with kidney-yang deficiency in rats were higher than those in the simple myocardial infarction models,but there were no significant differences between them(P>0.05).Conclusion The obvious differences are not showed in the parameters of the indexes of MIS,the myocardium enzyme in the serum,HCT and ESR,the indexes of the function of the blood plate,the weight of the thromb in vitro and the thrombelastogram.