喉鳞状细胞癌组织中Snail和E-cadherin的表达及其意义

目的:观察Snail和E-cadherin在喉鳞状细胞癌（LSCC）和癌旁组织中的表达情况，阐明两者与LSCC发生、发展的关系。方法：采用免疫组织化学染色技术分别检测46例患者的LSCC组织及癌旁组织中Snail和E-cadherin的表达，并将两者在LSCC中的表达情况与患者的临床病理特征进行比较，分析两者在LSCC组织中表达的相关性。结果：①Snail在LSCC和癌旁组织中表达的阳性率分别为69.57%（32/46）和36.96%（17/46），在LSCC组织中的表达阳性率明显高于癌旁组织（P＜0.05）；Snail在LSCC低分化组的表达阳性率明显高于高分化组和中分化组（P<0.05）。Snail的表达与LSCC的颈淋巴结转移及组织分化程度有关（P<0.05）。②E-cadherin在LSCC和癌旁组织中表达阳性率分别为47.83％（22/46）和78.30％（36/46），在LSCC组织中的表达阳性率明显低于癌旁组织（P＜0.05）；E-cadherin在LSCC低分化组的表达阳性率高于高分化组和中分化组（P<0.05）。E-cadherin的表达与LSCC的组织分化程度、T分级、颈淋巴结转移及临床分期有关联（P<0.01）。③LSCC组织中Snail和E-cadherin的表达呈负相关（r=－0.569 0，P<0.01）。结论：Snail和E-cadherin可能与LSCC的发生、发展密切相关，可作为判断LSCC恶性程度和预后的参考指标。

Expressions of Snail and E-cadherin in laryngeal squamous cell carcinoma tissues and their significances

Objective To observe the expressions of Snail and E-cadherin in laryngeal squamous cell carcinoma(LSCC) tissues and normal squamous epidermis tissues near cancer,and to clarify the relationship with the occurrence and development of LSCC.Methods Streptavidin peroxidase(S-P method) immunohistochemistry staining was used to detect the expressions of Snail and E-cadherin in 46 samples of LSCC and 46 samples of normal squamous epidermis near cancer.The relationship between the expressions of Snail and E-cadherin and the clinical pathological characteristics were analyzed.And the relevance of the expressions of Snail and E-cadherin in LSCC was also analyzed.Results ① The positive rates of Snail expressions in LSCC and normal squamous epidermis near cancer were 69.57%(32/46) and 36.96%(17/46).The expression of Snail in LSCC was significantly higher than that in normal squamous epidermis near cancer(P<0.05).There was significant correlation between the expression of Snail in LSCC and the differentiation and cervical lymph node matastasis(P<0.05).②The positive rates of E-cadherin expressions in LSCC and normal squamous epidermis near cancer were 47.83%(22/46) and 78.30%(36/46).The expression of E-cadherin in LSCC was significantly lower than that in normal squamous epidermis near cancer(P<0.05).There was significant correlation between the expression of E-cadherin in LSCC with the T stage,clinical stage,cervical lymph node matastasis and differentiation(P<0.05).③The expression of Snail in LSCC tissues was negatively correlated with the expression of Ecadherin(r=-0.569 0,P<0.01).Conclusion The expressions of Snail and E-cadherin may be associated with the occurrence and development of LSCC,and they could be used as the parameters which predict the malignant degree and prognosis of LSCC.