正交试验优选咽炎宁颗粒提取工艺

目的优选咽炎宁颗粒的提取工艺。方法应用高效液相法测定射干苷的含量;采用正交设计法考察煎煮次数、煎煮时间、加水量三因素对水提工艺提取成分提取率的影响;考察醇沉浓度、醇沉前水提液相对密度、醇沉时间三因素对醇沉工艺有效成分含量的影响。结果加水量对水提工艺有显著影响(P<0.05);醇沉浓度对醇沉工艺有显著影响(P<0.05)。结论最佳水提醇沉工艺为药材加10倍量水,煎煮2次,每次1.5 h;水提液浓缩至相对密度为1.15,加入乙醇使含醇量至60%,静置48 h。

Optimization of the extraction technology of yanyanning granule

Objective To optimize the extraction technology of yanyanning granule. Methods The content of tectoridin was detected by HPLC. Effects of three factors-extraction time, extraction times, water volume on extraction rate were evaluated by orthogonal design. Effects of three factors-alcohol precipitation concentration, concentration of condensed solution, precipitation time on content of tectoridin of alcohol precipitation were also evaluated by orthogonal design. Results Water volume had a significant effect on water-extraction（ P 〈 0.05 ） ; concentration of condensed solution had a significant effect on alcohol-precipitation （ P 〈 0.05 ）. Conclusion The optimized extraction technology is as follows ： 10 volumes of water are added to 1 volume of the crude drug, followed by decoction for 2 times, each de- coction lasting 1.5 h. The solution of water-extraction is concentrated to a relative density of 1.15 and then precipitated by alcohol at the concentration of 60% ,then keeping still for 48 h.